Key features and details
- Mouse polyclonal to MGEA5/OGA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MGEA5/OGA antibody
See all MGEA5/OGA primary antibodies
DescriptionMouse polyclonal to MGEA5/OGA
Species reactivityReacts with: Human
Full length protein corresponding to Human MGEA5/OGA.
Database link: NP_036347.1
- MGEA5/OGA transfected 293T cell lysate.
Previously labelled as MGEA5
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Concentration information loading...
PurityProtein G purified
FunctionIsoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).
Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
Tissue specificityUbiquitous. Shows highest expression in the brain, placenta and pancreas.
Sequence similaritiesBelongs to the glycosyl hydrolase 84 family.
modificationsProteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity.
Cellular localizationCytoplasm and Nucleus.
- Information by UniProt
- Beta-hexosaminidase antibody
- Beta-N-acetylglucosaminidase antibody
- Beta-N-acetylhexosaminidase antibody
Anti-MGEA5/OGA antibody (ab68522) at 1/2000 dilution + 293T Cell Lysate at 10 µg with 3% Milk
HRP conjugated Rat anti-mouse polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
ab68522 has been referenced in 1 publication.
- Lynch TP et al. Critical role of O-Linked ß-N-acetylglucosamine transferase in prostate cancer invasion, angiogenesis, and metastasis. J Biol Chem 287:11070-81 (2012). WB ; Human . PubMed: 22275356