Overview

  • Product name

  • Description

    Rabbit polyclonal to mGluR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IHC-Fr, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Synthetic peptide corresponding to Human mGluR1 aa 1159-1171.
    Sequence:

    SSVPSSPVSESVL

  • Positive control

    • Human brain lysate, rat dorsal root ganglion, and rat brain (cingulate cortex).
  • General notes

     This product was previously labelled as Metabotropic Glutamate Receptor 1, Metabotropic Glutamate Receptor 1a

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 133 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/500.
IHC-Fr 1/200.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function

    Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glutamate receptor metabotropic 1 antibody
    • glutamate receptor, metabotropic 1 antibody
    • GPRC1A antibody
    • GRM1 antibody
    • GRM1-Alpha antibody
    • GRM1_HUMAN antibody
    • GRM1A antibody
    • Metabotropic glutamate receptor 1 antibody
    • mGlu1 antibody
    • mGluR1 antibody
    • MGLUR1-Alpha antibody
    • MGLUR1A antibody
    • SCAR13 antibody
    see all

Images

  • Rat dorsal root ganglion. Tissue was fixed in 4% paraformaldehyde and cut into 10 micron sections. ab82211 was used at a dilution of 1/200 and secondary antibody at a dilution of 1/5000.
  • Anti-mGluR1 antibody (ab82211) at 1/1000 dilution + human brain lysate at 20 µg

    Secondary
    AP-conjugated secondary at 1/5000 dilution

    Predicted band size: 133 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescence labeling of mGluR1 in rat brain (cingulate cortex). Using ab82211 (red color). DAPI was used to counter-stain cell nuclei (blue color). Working dilution is 1/100-1/500.

  • ab82211 staining mGluR1 in rat cerebellum tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in paraformaldehyde, permeabilized using Triton X-100, blocked with 7% serum for 3 hours at 25°C, then incubated with ab82211 at a 1/200 dilution for 10 hours at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.

    See Abreview

References

This product has been referenced in:

  • Dubovyk V & Manahan-Vaughan D Less means more: The magnitude of synaptic plasticity along the hippocampal dorso-ventral axis is inversely related to the expression levels of plasticity-related neurotransmitter receptors. Hippocampus 28:136-150 (2018). WB, IHC-P ; Rat . Read more (PubMed: 29171922) »
  • Wang X  et al. Expression levels of long non-coding RNA HOXA distal transcript antisense RNA and metabotropic glutamate receptor 1 in pancreatic carcinoma, and their prognostic values. Oncol Lett 15:9464-9470 (2018). Read more (PubMed: 29844833) »
See all 5 Publications for this product

Customer reviews and Q&As

1-10 of 17 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (brainstem)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Permeabilization
Yes - 0.3% triton
Specification
brainstem
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 07 2013

Application
Immunohistochemistry (Frozen sections)
Blocking step
(agent) for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Sample
Cat Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 07 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (brainstem)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Permeabilization
Yes - 0.1% triton
Specification
brainstem
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Dr. Yuhua Liu

Verified customer

Submitted Sep 16 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Mouse Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 16 2013

Application
Immunocytochemistry
Sample
Rat Cell (brain)
Permeabilization
Yes - 0.3% triton
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted Sep 13 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Chicken Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% trition
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 11 2013

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brainstem)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Permeabilization
No
Specification
brainstem
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Wei Shi

Verified customer

Submitted Jul 16 2013

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Mouse Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 16 2013

Question
Answer

I performed a search of some other anti - metabotropic glutamate receptor 1 antibodies, and it seems the glutamate receptor can run between ˜110 - 130 kDa depending on the post-translational modification in the tested samples. Thus, the 110 kDa band that is observed in the western blot image on the datasheet for ab82211 represents metabotropic glutamate receptor 1.

Read More

Question
Answer

Thank you very much for your email.

Please do not hesitate to contact me if this antibody does not work.

I hope this information will be helpful. Should you have nay question please do not hesitate to contact me.

Read More

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up