Anti-mGluR1 antibody (ab82211)
Key features and details
- Rabbit polyclonal to mGluR1
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-mGluR1 antibody
See all mGluR1 primary antibodies -
Description
Rabbit polyclonal to mGluR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human mGluR1 aa 1159-1171.
Sequence:SSVPSSPVSESVL
Database link: Q13255 -
Positive control
- WB: Human brain lysate. ICC/IF: Rat brain (cingulate cortex).
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General notes
This product was previously labelled as Metabotropic Glutamate Receptor 1, Metabotropic Glutamate Receptor 1a.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab82211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 133 kDa).
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| ICC/IF |
1/100 - 1/500.
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| Notes |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 133 kDa). |
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ICC/IF
1/100 - 1/500. |
Target
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Function
Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. -
Sequence similarities
Belongs to the G-protein coupled receptor 3 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2911 Human
- Entrez Gene: 24414 Rat
- Omim: 604473 Human
- SwissProt: Q13255 Human
- SwissProt: P23385 Rat
- Unigene: 32945 Human
- Unigene: 87787 Rat
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Alternative names
- Glutamate receptor metabotropic 1 antibody
- glutamate receptor, metabotropic 1 antibody
- GPRC1A antibody
see all
Images
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Western blot - Anti-mGluR1 antibody (ab82211)Anti-mGluR1 antibody (ab82211) at 1/1000 dilution + human brain lysate at 20 µg
Secondary
AP-conjugated secondary at 1/5000 dilution
Predicted band size: 133 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-mGluR1 antibody (ab82211)Immunofluorescence labeling of mGluR1 in rat brain (cingulate cortex). Using ab82211 (red color). DAPI was used to counter-stain cell nuclei (blue color). Working dilution is 1/100-1/500.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab82211 has been referenced in 9 publications.
- Fan YN et al. Characterization of Group I Metabotropic Glutamate Receptors in Rat and Human Adrenal Glands. Front Physiol 11:401 (2020). PubMed: 32536873
- Beckmann D et al. Hippocampal Synaptic Plasticity, Spatial Memory, and Neurotransmitter Receptor Expression Are Profoundly Altered by Gradual Loss of Hearing Ability. Cereb Cortex 30:4581-4596 (2020). PubMed: 32202614
- Dubovyk V & Manahan-Vaughan D Distinct Time-Course of Alterations of Groups I and II Metabotropic Glutamate Receptor and GABAergic Receptor Expression Along the Dorsoventral Hippocampal Axis in an Animal Model of Psychosis. Front Behav Neurosci 13:98 (2019). PubMed: 31139061
- Lu Y et al. Activity-dependent synaptic integration and modulation of bilateral excitatory inputs in an auditory coincidence detection circuit. J Physiol 596:1981-1997 (2018). PubMed: 29572827
- Dubovyk V & Manahan-Vaughan D Less means more: The magnitude of synaptic plasticity along the hippocampal dorso-ventral axis is inversely related to the expression levels of plasticity-related neurotransmitter receptors. Hippocampus 28:136-150 (2018). WB, IHC-P ; Rat . PubMed: 29171922
- Wang X et al. Expression levels of long non-coding RNA HOXA distal transcript antisense RNA and metabotropic glutamate receptor 1 in pancreatic carcinoma, and their prognostic values. Oncol Lett 15:9464-9470 (2018). PubMed: 29844833
- Lin JJ et al. Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice. Theranostics 7:2015-2032 (2017). WB . PubMed: 28656058
- Valiya Veettil M et al. Glutamate secretion and metabotropic glutamate receptor 1 expression during Kaposi's sarcoma-associated herpesvirus infection promotes cell proliferation. PLoS Pathog 10:e1004389 (2014). Human . PubMed: 25299066
- Sylantyev S et al. Spike-driven glutamate electrodiffusion triggers synaptic potentiation via a homer-dependent mGluR-NMDAR link. Neuron 77:528-41 (2013). IHC-P . PubMed: 23395378
Customer reviews and Q&As
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