Anti-mGluR1 antibody (ab82211)
Key features and details
- Rabbit polyclonal to mGluR1
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-mGluR1 antibody
See all mGluR1 primary antibodies -
Description
Rabbit polyclonal to mGluR1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatWB Human
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Immunogen
Synthetic peptide corresponding to Human mGluR1 aa 1159-1171.
Sequence:SSVPSSPVSESVL
Database link: Q13255 -
Positive control
- WB: Human brain lysate. ICC/IF: Rat brain (cingulate cortex).
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General notes
This product was previously labelled as Metabotropic Glutamate Receptor 1, Metabotropic Glutamate Receptor 1a.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab82211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Rat
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 133 kDa).
|
|
| ICC/IF |
1/100 - 1/500.
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 133 kDa). |
|
ICC/IF
1/100 - 1/500. |
Target
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Function
Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. -
Sequence similarities
Belongs to the G-protein coupled receptor 3 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2911 Human
- Entrez Gene: 24414 Rat
- Omim: 604473 Human
- SwissProt: Q13255 Human
- SwissProt: P23385 Rat
- Unigene: 32945 Human
- Unigene: 87787 Rat
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Alternative names
- Glutamate receptor metabotropic 1 antibody
- glutamate receptor, metabotropic 1 antibody
- GPRC1A antibody
see all
Images
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Western blot - Anti-mGluR1 antibody (ab82211)Anti-mGluR1 antibody (ab82211) at 1/1000 dilution + human brain lysate at 20 µg
Secondary
AP-conjugated secondary at 1/5000 dilution
Predicted band size: 133 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-mGluR1 antibody (ab82211)Immunofluorescence labeling of mGluR1 in rat brain (cingulate cortex). Using ab82211 (red color). DAPI was used to counter-stain cell nuclei (blue color). Working dilution is 1/100-1/500.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab82211 has been referenced in 9 publications.
- Fan YN et al. Characterization of Group I Metabotropic Glutamate Receptors in Rat and Human Adrenal Glands. Front Physiol 11:401 (2020). PubMed: 32536873
- Beckmann D et al. Hippocampal Synaptic Plasticity, Spatial Memory, and Neurotransmitter Receptor Expression Are Profoundly Altered by Gradual Loss of Hearing Ability. Cereb Cortex 30:4581-4596 (2020). PubMed: 32202614
- Dubovyk V & Manahan-Vaughan D Distinct Time-Course of Alterations of Groups I and II Metabotropic Glutamate Receptor and GABAergic Receptor Expression Along the Dorsoventral Hippocampal Axis in an Animal Model of Psychosis. Front Behav Neurosci 13:98 (2019). PubMed: 31139061
- Lu Y et al. Activity-dependent synaptic integration and modulation of bilateral excitatory inputs in an auditory coincidence detection circuit. J Physiol 596:1981-1997 (2018). PubMed: 29572827
- Dubovyk V & Manahan-Vaughan D Less means more: The magnitude of synaptic plasticity along the hippocampal dorso-ventral axis is inversely related to the expression levels of plasticity-related neurotransmitter receptors. Hippocampus 28:136-150 (2018). WB, IHC-P ; Rat . PubMed: 29171922
- Wang X et al. Expression levels of long non-coding RNA HOXA distal transcript antisense RNA and metabotropic glutamate receptor 1 in pancreatic carcinoma, and their prognostic values. Oncol Lett 15:9464-9470 (2018). PubMed: 29844833
- Lin JJ et al. Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice. Theranostics 7:2015-2032 (2017). WB . PubMed: 28656058
- Valiya Veettil M et al. Glutamate secretion and metabotropic glutamate receptor 1 expression during Kaposi's sarcoma-associated herpesvirus infection promotes cell proliferation. PLoS Pathog 10:e1004389 (2014). Human . PubMed: 25299066
- Sylantyev S et al. Spike-driven glutamate electrodiffusion triggers synaptic potentiation via a homer-dependent mGluR-NMDAR link. Neuron 77:528-41 (2013). IHC-P . PubMed: 23395378
Customer reviews and Q&As
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