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    mglur1-antibody-ab82211.pdf

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Neuroscience Neurotransmission Receptors / Channels GPCR Glutamate Receptors
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Anti-mGluR1 antibody (ab82211)

  • Datasheet
  • SDS
Reviews (14)Q&A (3)References (9)

Product price, shipping and contact information

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Western blot - Anti-mGluR1 antibody (ab82211)
  • Immunocytochemistry/ Immunofluorescence - Anti-mGluR1 antibody (ab82211)

Key features and details

  • Rabbit polyclonal to mGluR1
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-mGluR1a antibody [EPR13540] (ab183712)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-mGluR1 antibody
    See all mGluR1 primary antibodies
  • Description

    Rabbit polyclonal to mGluR1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Rat
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human mGluR1 aa 1159-1171.
    Sequence:

    SSVPSSPVSESVL


    Database link: Q13255
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Human brain lysate. ICC/IF: Rat brain (cingulate cortex).
  • General notes

    This product was previously labelled as Metabotropic Glutamate Receptor 1, Metabotropic Glutamate Receptor 1a.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Glutamate Receptors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human mGluR1 protein (ab114707)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab82211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Rat
WB
Human
Application Abreviews Notes
WB
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 133 kDa).
ICC/IF
1/100 - 1/500.
Notes
WB
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 133 kDa).
ICC/IF
1/100 - 1/500.

Target

  • Function

    Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q13255 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2911 Human
    • Entrez Gene: 24414 Rat
    • Omim: 604473 Human
    • SwissProt: Q13255 Human
    • SwissProt: P23385 Rat
    • Unigene: 32945 Human
    • Unigene: 87787 Rat
    • Alternative names

      • Glutamate receptor metabotropic 1 antibody
      • glutamate receptor, metabotropic 1 antibody
      • GPRC1A antibody
      • GRM1 antibody
      • GRM1-Alpha antibody
      • GRM1_HUMAN antibody
      • GRM1A antibody
      • Metabotropic glutamate receptor 1 antibody
      • mGlu1 antibody
      • mGluR1 antibody
      • MGLUR1-Alpha antibody
      • MGLUR1A antibody
      • SCAR13 antibody
      see all

    Images

    • Western blot - Anti-mGluR1 antibody (ab82211)
      Western blot - Anti-mGluR1 antibody (ab82211)
      Anti-mGluR1 antibody (ab82211) at 1/1000 dilution + human brain lysate at 20 µg

      Secondary
      AP-conjugated secondary at 1/5000 dilution

      Predicted band size: 133 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-mGluR1 antibody (ab82211)
      Immunocytochemistry/ Immunofluorescence - Anti-mGluR1 antibody (ab82211)

      Immunofluorescence labeling of mGluR1 in rat brain (cingulate cortex). Using ab82211 (red color). DAPI was used to counter-stain cell nuclei (blue color). Working dilution is 1/100-1/500.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (9)

    Publishing research using ab82211? Please let us know so that we can cite the reference in this datasheet.

    ab82211 has been referenced in 9 publications.

    • Fan YN  et al. Characterization of Group I Metabotropic Glutamate Receptors in Rat and Human Adrenal Glands. Front Physiol 11:401 (2020). PubMed: 32536873
    • Beckmann D  et al. Hippocampal Synaptic Plasticity, Spatial Memory, and Neurotransmitter Receptor Expression Are Profoundly Altered by Gradual Loss of Hearing Ability. Cereb Cortex 30:4581-4596 (2020). PubMed: 32202614
    • Dubovyk V & Manahan-Vaughan D Distinct Time-Course of Alterations of Groups I and II Metabotropic Glutamate Receptor and GABAergic Receptor Expression Along the Dorsoventral Hippocampal Axis in an Animal Model of Psychosis. Front Behav Neurosci 13:98 (2019). PubMed: 31139061
    • Lu Y  et al. Activity-dependent synaptic integration and modulation of bilateral excitatory inputs in an auditory coincidence detection circuit. J Physiol 596:1981-1997 (2018). PubMed: 29572827
    • Dubovyk V & Manahan-Vaughan D Less means more: The magnitude of synaptic plasticity along the hippocampal dorso-ventral axis is inversely related to the expression levels of plasticity-related neurotransmitter receptors. Hippocampus 28:136-150 (2018). WB, IHC-P ; Rat . PubMed: 29171922
    • Wang X  et al. Expression levels of long non-coding RNA HOXA distal transcript antisense RNA and metabotropic glutamate receptor 1 in pancreatic carcinoma, and their prognostic values. Oncol Lett 15:9464-9470 (2018). PubMed: 29844833
    • Lin JJ  et al. Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice. Theranostics 7:2015-2032 (2017). WB . PubMed: 28656058
    • Valiya Veettil M  et al. Glutamate secretion and metabotropic glutamate receptor 1 expression during Kaposi's sarcoma-associated herpesvirus infection promotes cell proliferation. PLoS Pathog 10:e1004389 (2014). Human . PubMed: 25299066
    • Sylantyev S  et al. Spike-driven glutamate electrodiffusion triggers synaptic potentiation via a homer-dependent mGluR-NMDAR link. Neuron 77:528-41 (2013). IHC-P . PubMed: 23395378

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    11-17 of 17 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mGluR1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Meriones unguiculatus Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.3% Triton, 0.1% Saponin in 0.1 M PBS
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 06 2012

    Question

    Do you have blocking peptides for these MGLUR2/3 and MGLUR1 antibodies?

    Read More

    Abcam community

    Verified customer

    Asked on May 23 2012

    Answer

    Thank you for your phone call.
    Unfortunately, the blocking peptides for these antibodies are not available for sale through Abcam. You may be able to find existing peptides on biocompare.com, or have them synthesized using the following sequences:
    ab6438: NGREVVDSTTSSL
    ab82211: SSVPSSPVSESVL
    I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Abcam Scientific Support

    Answered on May 23 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-mGluR1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (cerebellum)
    Permeabilization
    No
    Specification
    cerebellum
    Blocking step
    Milk as blocking agent for 4 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 25 2011

    Immunohistochemistry (Frozen sections) abreview for Anti-mGluR1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Chicken Tissue sections (brainstem)
    Permeabilization
    Yes - Triton X-100
    Specification
    brainstem
    Blocking step
    Serum as blocking agent for 5 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 24 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Metabotropic Glutamate Receptor 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Chicken Tissue sections (brainstem)
    Specification
    brainstem
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate sodium
    Blocking step
    Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 24 2011

    Immunohistochemistry (Frozen sections) abreview for Anti-mGluR1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (cerebellum)
    Permeabilization
    Yes - Triton X-100
    Specification
    cerebellum
    Blocking step
    Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 24 2011

    Immunohistochemistry (Frozen sections) abreview for Anti-Metabotropic Glutamate Receptor 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Chicken Tissue sections (brainstem)
    Specification
    brainstem
    Fixative
    Formaldehyde
    Permeabilization
    Yes - Triton X-100
    Blocking step
    Serum as blocking agent for 3 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 24 2011

    11-17 of 17 Abreviews or Q&A

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