Anti-mGluR1 antibody (ab82211)
- Datasheet
- References (5)
- Protocols
Overview
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Product name
Anti-mGluR1 antibody
See all mGluR1 primary antibodies -
Description
Rabbit polyclonal to mGluR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IHC-Fr, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Human -
Immunogen
Synthetic peptide corresponding to Human mGluR1 aa 1159-1171.
Sequence:SSVPSSPVSESVL
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Positive control
- Human brain lysate, rat dorsal root ganglion, and rat brain (cingulate cortex).
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General notes
This product was previously labelled as Metabotropic Glutamate Receptor 1, Metabotropic Glutamate Receptor 1a
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab82211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 133 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | 1/100 - 1/500. | |
| IHC-Fr | 1/200. | |
| IHC-FoFr | Use at an assay dependent concentration. |
Target
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Function
Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. -
Sequence similarities
Belongs to the G-protein coupled receptor 3 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2911 Human
- Entrez Gene: 14816 Mouse
- Entrez Gene: 24414 Rat
- Omim: 604473 Human
- SwissProt: Q13255 Human
- SwissProt: P97772 Mouse
- SwissProt: P23385 Rat
- Unigene: 32945 Human
see all -
Alternative names
- Glutamate receptor metabotropic 1 antibody
- glutamate receptor, metabotropic 1 antibody
- GPRC1A antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mGluR1 antibody (ab82211)Rat dorsal root ganglion. Tissue was fixed in 4% paraformaldehyde and cut into 10 micron sections. ab82211 was used at a dilution of 1/200 and secondary antibody at a dilution of 1/5000. -
Western blot - Anti-mGluR1 antibody (ab82211)Anti-mGluR1 antibody (ab82211) at 1/1000 dilution + human brain lysate at 20 µg
Secondary
AP-conjugated secondary at 1/5000 dilution
Predicted band size: 133 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-mGluR1 antibody (ab82211)Immunofluorescence labeling of mGluR1 in rat brain (cingulate cortex). Using ab82211 (red color). DAPI was used to counter-stain cell nuclei (blue color). Working dilution is 1/100-1/500.
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Immunohistochemistry (Frozen sections) - Anti-mGluR1 antibody (ab82211)Image courtesy of an anonymous Abreview.ab82211 staining mGluR1 in rat cerebellum tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in paraformaldehyde, permeabilized using Triton X-100, blocked with 7% serum for 3 hours at 25°C, then incubated with ab82211 at a 1/200 dilution for 10 hours at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Dubovyk V & Manahan-Vaughan D Less means more: The magnitude of synaptic plasticity along the hippocampal dorso-ventral axis is inversely related to the expression levels of plasticity-related neurotransmitter receptors. Hippocampus 28:136-150 (2018). WB, IHC-P ; Rat . Read more (PubMed: 29171922) »
- Wang X et al. Expression levels of long non-coding RNA HOXA distal transcript antisense RNA and metabotropic glutamate receptor 1 in pancreatic carcinoma, and their prognostic values. Oncol Lett 15:9464-9470 (2018). Read more (PubMed: 29844833) »
