Anti-mGluR1 antibody (ab82211)

Reviews (14)Q&A (3)References (5)

Overview

  • Product name

    Anti-mGluR1 antibody
    See all mGluR1 primary antibodies

  • Description

    Rabbit polyclonal to mGluR1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IHC-Fr, IHC-FoFrmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Human

  • Immunogen

    Synthetic peptide corresponding to Human mGluR1 aa 1159-1171.
    Sequence:

    SSVPSSPVSESVL

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human brain lysate, rat dorsal root ganglion, and rat brain (cingulate cortex).

  • General notes

     This product was previously labelled as Metabotropic Glutamate Receptor 1, Metabotropic Glutamate Receptor 1a

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 133 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/500.
IHC-Fr 1/200.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function

    Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Glutamate receptor metabotropic 1 antibody
    • glutamate receptor, metabotropic 1 antibody
    • GPRC1A antibody
    • GRM1 antibody
    • GRM1-Alpha antibody
    • GRM1_HUMAN antibody
    • GRM1A antibody
    • Metabotropic glutamate receptor 1 antibody
    • mGlu1 antibody
    • mGluR1 antibody
    • MGLUR1-Alpha antibody
    • MGLUR1A antibody
    • SCAR13 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mGluR1 antibody (ab82211)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mGluR1 antibody (ab82211)
    Rat dorsal root ganglion. Tissue was fixed in 4% paraformaldehyde and cut into 10 micron sections. ab82211 was used at a dilution of 1/200 and secondary antibody at a dilution of 1/5000.
  • Western blot - Anti-mGluR1 antibody (ab82211)
    Western blot - Anti-mGluR1 antibody (ab82211)
    Anti-mGluR1 antibody (ab82211) at 1/1000 dilution + human brain lysate at 20 µg

    Secondary
    AP-conjugated secondary at 1/5000 dilution

    Predicted band size: 133 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-mGluR1 antibody (ab82211)
    Immunocytochemistry/ Immunofluorescence - Anti-mGluR1 antibody (ab82211)

    Immunofluorescence labeling of mGluR1 in rat brain (cingulate cortex). Using ab82211 (red color). DAPI was used to counter-stain cell nuclei (blue color). Working dilution is 1/100-1/500.

  • Immunohistochemistry (Frozen sections) - Anti-mGluR1 antibody (ab82211)
    Immunohistochemistry (Frozen sections) - Anti-mGluR1 antibody (ab82211)Image courtesy of an anonymous Abreview.

    ab82211 staining mGluR1 in rat cerebellum tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in paraformaldehyde, permeabilized using Triton X-100, blocked with 7% serum for 3 hours at 25°C, then incubated with ab82211 at a 1/200 dilution for 10 hours at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.

    See Abreview

References

This product has been referenced in:

  • Dubovyk V & Manahan-Vaughan D Less means more: The magnitude of synaptic plasticity along the hippocampal dorso-ventral axis is inversely related to the expression levels of plasticity-related neurotransmitter receptors. Hippocampus 28:136-150 (2018). WB, IHC-P ; Rat . Read more (PubMed: 29171922) »
  • Wang X  et al. Expression levels of long non-coding RNA HOXA distal transcript antisense RNA and metabotropic glutamate receptor 1 in pancreatic carcinoma, and their prognostic values. Oncol Lett 15:9464-9470 (2018). Read more (PubMed: 29844833) »
See all 5 Publications for this product

Publishing research using ab82211? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Q&A

Question

ab82211 is interested in it, but the WB band is not at the correct spot

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Answer

I performed a search of some other anti - metabotropic glutamate receptor 1 antibodies, and it seems the glutamate receptor can run between ˜110 - 130 kDa depending on the post-translational modification in the tested samples. Thus, the 110 kDa band that is observed in the western blot image on the datasheet for ab82211 represents metabotropic glutamate receptor 1.

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Question

Would like use in mouse bone sections in IHC-P or ICC/IF.

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Answer

Thank you very much for your email.

Please do not hesitate to contact me if this antibody does not work.

I hope this information will be helpful. Should you have nay question please do not hesitate to contact me.

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Question

Do you have blocking peptides for these MGLUR2/3 and MGLUR1 antibodies?

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Answer

Thank you for your phone call.
Unfortunately, the blocking peptides for these antibodies are not available for sale through Abcam. You may be able to find existing peptides on biocompare.com, or have them synthesized using the following sequences:
ab6438: NGREVVDSTTSSL
ab82211: SSVPSSPVSESVL
I hope this helps, please let me know if you need any additional information or assistance.

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