Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Metabotropic Glutamate Receptor 1 antibody

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Mouse Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde

Other product details

Incubation time
8 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: mocular probes
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

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Submitted Jul 16 2013

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