Anti-mGluR1a antibody (ab51314)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-mGluR1a antibody
See all mGluR1a primary antibodies -
Description
Rabbit polyclonal to mGluR1a -
Host species
Rabbit -
Specificity
IHC-P results for Mouse have been shown to be negative for this antibody. Although, there is significant homology with Mouse, it is not predicted species. -
Tested applications
Suitable for: ICC/IF, WB, IHC (PFA fixed)more details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Cynomolgus monkey
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Immunogen
Synthetic peptide corresponding to Rat mGluR1a aa 1159-1171.
Sequence:SSVPSSPVSESVL
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Positive control
- Rat brain (singulate cortex) Human brain lysate Rat cerebellar lysates
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General notes
This product was previously labelled as Metabotropic Glutamate Receptor 1, Metabotropic Glutamate Receptor 1a
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab51314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| WB | ||
| IHC (PFA fixed) |
Application notes
ICC/IF: 1/250.
Immunohistochemistry (PFA fixed): 1/250.
WB: 1/1000. Predicted molecular weight: 133 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry (PFA fixed): 1/250.
WB: 1/1000. Predicted molecular weight: 133 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. -
Tissue specificity
Detected in brain. -
Involvement in disease
Spinocerebellar ataxia, autosomal recessive, 13 -
Sequence similarities
Belongs to the G-protein coupled receptor 3 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2911 Human
- Entrez Gene: 24414 Rat
- Omim: 604473 Human
- SwissProt: Q13255 Human
- SwissProt: P23385 Rat
- Unigene: 32945 Human
- Unigene: 87787 Rat
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Alternative names
- Glutamate receptor metabotropic 1 antibody
- GPRC1A antibody
- GRM1 alpha antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-mGluR1a antibody (ab51314)mGluR1a staining in rat brain (singulate cortex) using ab51314 at 1/100 dilution. mGluR1a detection was done using anti-rabbit Cy3 conjugated antibodies (red color). DAPI was used to counterstain cell nuclei (blue color).
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Western blot - Anti-mGluR1a antibody (ab51314)Anti-mGluR1a antibody (ab51314) at 1/1000 dilution + Human brain lysate at 20 µg
Secondary
AP-conjugated secondary at 1/5000 dilution
Predicted band size: 133 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted? -
Western blot - Anti-mGluR1a antibody (ab51314)All lanes : Anti-mGluR1a antibody (ab51314)
Lane 1 : Rat cerebellar membranes
Lane 2 : Rat cerebellar membranes + DTT
Predicted band size: 133 kDa
Observed band size: 133 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Gruol DL et al. Neuroadaptive changes in cerebellar neurons induced by chronic exposure to IL-6. J Neuroimmunol 239:28-36 (2011). WB ; Mouse . Read more (PubMed: 21890220) »
- Romano C et al. Distribution of metabotropic glutamate receptor mGluR5 immunoreactivity in rat brain. J Comp Neurol 355:455-69 (1995). Read more (PubMed: 7636025) »
