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    mglur1a-antibody-ab51314.pdf

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Neuroscience Neurotransmission Receptors / Channels GPCR Glutamate Receptors
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Anti-mGluR1a antibody (ab51314)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

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Immunocytochemistry/ Immunofluorescence - Anti-mGluR1a antibody (ab51314)
  • Western blot - Anti-mGluR1a antibody (ab51314)
  • Western blot - Anti-mGluR1a antibody (ab51314)

Key features and details

  • Rabbit polyclonal to mGluR1a
  • Suitable for: ICC/IF, WB
  • Reacts with: Rat, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-mGluR1a antibody
    See all mGluR1a primary antibodies
  • Description

    Rabbit polyclonal to mGluR1a
  • Host species

    Rabbit
  • Specificity

    IHC-P results for Mouse have been shown to be negative for this antibody. Although, there is significant homology with Mouse, it is not predicted species.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Rat mGluR1a aa 1159-1171.
    Sequence:

    SSVPSSPVSESVL

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

     This product was previously labelled as Metabotropic Glutamate Receptor 1, Metabotropic Glutamate Receptor 1a

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Glutamate Receptors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/250.
WB (1)
1/1000. Predicted molecular weight: 133 kDa.
Notes
ICC/IF
1/250.
WB
1/1000. Predicted molecular weight: 133 kDa.

Target

  • Function

    G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum.
  • Tissue specificity

    Detected in brain.
  • Involvement in disease

    Spinocerebellar ataxia, autosomal recessive, 13
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q13255 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2911 Human
    • Entrez Gene: 24414 Rat
    • Omim: 604473 Human
    • SwissProt: Q13255 Human
    • SwissProt: P23385 Rat
    • Unigene: 32945 Human
    • Unigene: 87787 Rat
    • Alternative names

      • Glutamate receptor metabotropic 1 antibody
      • GPRC1A antibody
      • GRM1 alpha antibody
      • GRM1 antibody
      • GRM1_HUMAN antibody
      • Metabotropic glutamate receptor 1 antibody
      • MGLU1 antibody
      • mGluR1 antibody
      • SCAR13 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-mGluR1a antibody (ab51314)
      Immunocytochemistry/ Immunofluorescence - Anti-mGluR1a antibody (ab51314)

      mGluR1a staining in rat brain (singulate cortex) using ab51314 at 1/100 dilution. mGluR1a detection was done using anti-rabbit Cy3 conjugated antibodies (red color). DAPI was used to counterstain cell nuclei (blue color).

    • Western blot - Anti-mGluR1a antibody (ab51314)
      Western blot - Anti-mGluR1a antibody (ab51314)
      Anti-mGluR1a antibody (ab51314) at 1/1000 dilution + Human brain lysate at 20 µg

      Secondary
      AP-conjugated secondary at 1/5000 dilution

      Predicted band size: 133 kDa
      Observed band size: 105 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-mGluR1a antibody (ab51314)
      Western blot - Anti-mGluR1a antibody (ab51314)
      All lanes : Anti-mGluR1a antibody (ab51314)

      Lane 1 : Rat cerebellar membranes
      Lane 2 : Rat cerebellar membranes + DTT

      Predicted band size: 133 kDa
      Observed band size: 133 kDa

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab51314? Please let us know so that we can cite the reference in this datasheet.

    ab51314 has been referenced in 2 publications.

    • Gruol DL  et al. Neuroadaptive changes in cerebellar neurons induced by chronic exposure to IL-6. J Neuroimmunol 239:28-36 (2011). WB ; Mouse . PubMed: 21890220
    • Romano C  et al. Distribution of metabotropic glutamate receptor mGluR5 immunoreactivity in rat brain. J Comp Neurol 355:455-69 (1995). PubMed: 7636025

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Metabotropic Glutamate Receptor 1a antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Ferret Tissue lysate - whole (Parietal cortex in Brain)
    Gel Running Conditions
    Reduced Denaturing (Nupage 3-8% Tris-acetate gel)
    Loading amount
    40 µg
    Specification
    Parietal cortex in Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Francis Djankpa

    Verified customer

    Submitted Jan 07 2016

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