Immunocytochemistry/ Immunofluorescence abreview for Anti-MGMT antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (T98G human glioma cell line)
Specification
T98G human glioma cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TX-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C

Other product details

Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/300

Additional data

Additional Notes
Cells were fixed for 20 minutes using 4% paraformaldehyde/PBS.

MGM-T stain is shown in red (alexa 555) and stains nuclei and to a small extent, cytoplasm, nuclei were stained with DAPI (blue), while microtubules were stained using an anti-tubulin FITC conjugate (green).

Dr. Hilary Pollard

Verified customer

Submitted May 26 2011

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