Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-MGMT antibody [EPR4398] - BSA and Azide free (ab247735)

Overview

  • Product name
    Anti-MGMT antibody [EPR4398] - BSA and Azide free
    See all MGMT primary antibodies
  • Description
    Rabbit monoclonal [EPR4398] to MGMT - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MGMT aa 150 to the C-terminus. The exact sequence is proprietary.

  • General notes

    ab247735 is a PBS-only buffer format of ab108989. Please refer to ab108989 for recommended dilutions, protocols, and image data.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab247735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 23 kDa (predicted molecular weight: 22 kDa).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
    • Sequence similarities
      Belongs to the MGMT family.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 6 O methylguanine DNA methyltransferase antibody
      • 6-O-methylguanine-DNA methyltransferase antibody
      • Agat antibody
      • AGT antibody
      • AI267024 antibody
      • EC 2.1.1.63 antibody
      • Methylated DNA protein cysteine methyltransferase antibody
      • Methylated-DNA--protein-cysteine methyltransferase antibody
      • Methylguanine DNA methyltransferase antibody
      • MGC107020 antibody
      • MGMT antibody
      • MGMT_HUMAN antibody
      • O 6 methylguanine DNA alkyltransferase antibody
      • O 6 methylguanine DNA methyltransferase antibody
      • O-6-methylguanine-DNA methyltransferase antibody
      • O-6-methylguanine-DNA-alkyltransferase antibody
      see all

    References

    ab247735 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab247735.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
    For licensing inquiries, please contact partnerships@abcam.com

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