Product nameMGMT overexpression 293T lysate (whole cell)
ab94051 is a 293T cell transfected lysate in which Human MGMT has been transiently over-expressed using a pCMV-MGMT plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Similarity: Belongs to the MGMT family.
Our Abpromise guarantee covers the use of ab94051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94051 at 15µg/lane on an SDS-PAGE gel
All lanes : Anti-MGMT antibody (ab55442) at 1/500 dilution
Lane 1 :
MGMT overexpression 293T lysate (whole cell) (ab94051)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94051 has not yet been referenced specifically in any publications.