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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes RING Finger E3 Ligase
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Anti-MGRN1 antibody (ab101997)

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Immunoprecipitation - Anti-MGRN1 antibody (ab101997)

    Key features and details

    • Rabbit polyclonal to MGRN1
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human MGRN1 protein (ab167954)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-MGRN1 antibody
      See all MGRN1 primary antibodies
    • Description

      Rabbit polyclonal to MGRN1
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IPmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Chimpanzee, Gorilla
    • Immunogen

      Synthetic peptide corresponding to Human MGRN1 aa 505-555.
      Database link: NP_001135761.1

    • Positive control

      • IP: HeLa whole cell lysate.
    • General notes

      Previously labelled as RNF156. 

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: Tris citrate/phosphate
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Ubiquitin E3 Enzymes
      • RING Finger E3 Ligase

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa whole cell lysate (ab29545)
    • Recombinant Protein

      • Recombinant Human MGRN1 protein (ab167954)

    Applications

    Our Abpromise guarantee covers the use of ab101997 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP Use at 2-10 µg/mg of lysate.

    Target

    • Function

      E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Contains 1 RING-type zinc finger.
    • Domain

      The RING finger is required for ubiquitin ligase activity.
    • Post-translational
      modifications

      Autoubiquitinated in vitro.
    • Cellular localization

      Cytoplasm > cytosol. Cell membrane; Early endosome. The endosomal localization is dependent on the interaction with TSG101 and Cytoplasm > cytosol. Nucleus. Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.
    • Target information above from: UniProt accession O60291 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 23295 Human
      • Omim: 607559 Human
      • SwissProt: O60291 Human
      • Unigene: 526494 Human
      • Alternative names

        • E3 ubiquitin-protein ligase MGRN1 antibody
        • KIAA0544 antibody
        • Mahogunin ring finger 1 antibody
        • Mahogunin RING finger protein 1 antibody
        • MGRN 1 antibody
        • Mgrn1 antibody
        • MGRN1_HUMAN antibody
        • Probable E3 ubiquitin protein ligase MGRN1 antibody
        • RING finger protein 156 antibody
        • RNF 156 antibody
        • RNF156 antibody
        see all

      Images

      • Immunoprecipitation - Anti-MGRN1 antibody (ab101997)
        Immunoprecipitation - Anti-MGRN1 antibody (ab101997)

        MGRN1 was immunoprecipitated from HeLa cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab101997 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using an alternative anti-MGRN1 antibody at 0.4 µg/ml.

        Lane 1: Rabbit anti-MGRN1 Antibody IP in HeLa whole cell lysate.
        Lane 2: ab101997 IP in HeLa whole cell lysate. 
        Lane 3: Control IgG IP in HeLa whole cell lysate.

        Detection: Chemiluminescence with an exposure time of 10 seconds.

      Protocols

      • Immunoprecipitation protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab101997? Please let us know so that we can cite the reference in this datasheet.

      ab101997 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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