Anti-MGRN1 antibody (ab101997)
Key features and details
- Rabbit polyclonal to MGRN1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MGRN1 antibody
See all MGRN1 primary antibodies -
Description
Rabbit polyclonal to MGRN1 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla
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Immunogen
Synthetic peptide corresponding to Human MGRN1 aa 505-555.
Database link: NP_001135761.1 -
Positive control
- IP: HeLa whole cell lysate.
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General notes
Previously labelled as RNF156.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab101997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-type zinc finger. -
Domain
The RING finger is required for ubiquitin ligase activity. -
Post-translational
modificationsAutoubiquitinated in vitro. -
Cellular localization
Cytoplasm > cytosol. Cell membrane; Early endosome. The endosomal localization is dependent on the interaction with TSG101 and Cytoplasm > cytosol. Nucleus. Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli. - Information by UniProt
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Database links
- Entrez Gene: 23295 Human
- Omim: 607559 Human
- SwissProt: O60291 Human
- Unigene: 526494 Human
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Alternative names
- E3 ubiquitin-protein ligase MGRN1 antibody
- KIAA0544 antibody
- Mahogunin ring finger 1 antibody
see all
Images
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Immunoprecipitation - Anti-MGRN1 antibody (ab101997)
MGRN1 was immunoprecipitated from HeLa cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab101997 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using an alternative anti-MGRN1 antibody at 0.4 µg/ml.
Lane 1: Rabbit anti-MGRN1 Antibody IP in HeLa whole cell lysate.
Lane 2: ab101997 IP in HeLa whole cell lysate.
Lane 3: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 10 seconds.
Datasheets and documents
References (0)
ab101997 has not yet been referenced specifically in any publications.
