Key features and details
- Rabbit polyclonal to MGRN1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MGRN1 antibody
See all MGRN1 primary antibodies
DescriptionRabbit polyclonal to MGRN1
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide corresponding to Human MGRN1 aa 505-555.
Database link: NP_001135761.1
- IP: HeLa whole cell lysate.
Previously labelled as RNF156.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab101997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionE3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 RING-type zinc finger.
DomainThe RING finger is required for ubiquitin ligase activity.
modificationsAutoubiquitinated in vitro.
Cellular localizationCytoplasm > cytosol. Cell membrane; Early endosome. The endosomal localization is dependent on the interaction with TSG101 and Cytoplasm > cytosol. Nucleus. Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.
- Information by UniProt
- E3 ubiquitin-protein ligase MGRN1 antibody
- KIAA0544 antibody
- Mahogunin ring finger 1 antibody
MGRN1 was immunoprecipitated from HeLa cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab101997 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using an alternative anti-MGRN1 antibody at 0.4 µg/ml.
Lane 1: Rabbit anti-MGRN1 Antibody IP in HeLa whole cell lysate.
Lane 2: ab101997 IP in HeLa whole cell lysate.
Lane 3: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab101997 has not yet been referenced specifically in any publications.