Overview

  • Product name

  • Description

    Rabbit polyclonal to MGRN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human MGRN1 aa 67-294.
    Database link: O60291

  • Positive control

    • NT2D1 and IMR32 whole cell lysates.
  • General notes

    Previously labelled as RNF156. 

Properties

Applications

Our Abpromise guarantee covers the use of ab154056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 63 kDa.

Target

  • Function

    E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
  • Domain

    The RING finger is required for ubiquitin ligase activity.
  • Post-translational
    modifications

    Autoubiquitinated in vitro.
  • Cellular localization

    Cytoplasm > cytosol. Cell membrane; Early endosome. The endosomal localization is dependent on the interaction with TSG101 and Cytoplasm > cytosol. Nucleus. Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin-protein ligase MGRN1 antibody
    • KIAA0544 antibody
    • Mahogunin ring finger 1 antibody
    • Mahogunin RING finger protein 1 antibody
    • MGRN 1 antibody
    • Mgrn1 antibody
    • MGRN1_HUMAN antibody
    • Probable E3 ubiquitin protein ligase MGRN1 antibody
    • RING finger protein 156 antibody
    • RNF 156 antibody
    • RNF156 antibody
    see all

Images

  • Anti-MGRN1 antibody (ab154056) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size: 63 kDa



    7.5% SDS PAGE

References

ab154056 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary hippocampal neurons)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
Primary hippocampal neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 25 2014

Application
Western blot
Sample
Human Cell lysate - whole cell (Rat Primary hippocampal neurons Infected with Huma)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Treatment
Infected with Human MGRN-GFP
Specification
Rat Primary hippocampal neurons Infected with Huma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 25 2014

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