Key features and details
- Rabbit polyclonal to MGST1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-MGST1 antibody
See all MGST1 primary antibodies
DescriptionRabbit polyclonal to MGST1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee
Recombinant fragment within Human MGST1. The exact sequence is proprietary.
Database link: P10620
- WB: HeLa and HepG2 whole cell lysate. IHC: Mouse and rat liver tissue.
This product was previously labelled as Microsomal Glutathione S-transferase 1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 18 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionConjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.
Tissue specificityHighly expressed in liver.
Sequence similaritiesBelongs to the MAPEG family.
modificationsPeroxynitrite induces nitration at Tyr-93 which activates the enzyme.
Cellular localizationMicrosome. Mitochondrion outer membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- MGST1 antibody
- Glutathione S transferase 12 antibody
- GST12 antibody
All lanes :
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 18 kDa
Paraffin-embedded mouse liver tissue stained for MGST1 protein using ab228947 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded rat liver tissue stained for MGST1 protein using ab228947 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228947 has not yet been referenced specifically in any publications.