RecombinantRabMAb

Recombinant Anti-MGST1 antibody [EPR7934] (ab131059)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] (ab131059)
  • Western blot - Anti-MGST1 antibody [EPR7934] (ab131059)
  • Flow Cytometry - Anti-MGST1 antibody [EPR7934] (ab131059)
  • Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] (ab131059)
  • Western blot - Anti-MGST1 antibody [EPR7934] (ab131059)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] (ab131059)
  • OI-RD Scanning - Anti-MGST1 antibody [EPR7934] (ab131059)
  • Anti-MGST1 antibody [EPR7934] (ab131059)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7934] to MGST1
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MGST1 antibody [EPR7934]
    See all MGST1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7934] to MGST1

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human MGST1 aa 1-100.
    Database link: P10620

  • Positive control

    • Human fetal liver, mouse liver, HepG2, and HT-1376 lysates; Human liver tissue. MCF7

  • General notes

     This product was previously labelled as Microsomal Glutathione S-transferase 1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/50 - 1/100. 
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF
1/50 - 1/100.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/50 - 1/100. 
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF
1/50 - 1/100.

Target

  • Function

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.

  • Tissue specificity

    Highly expressed in liver.

  • Sequence similarities

    Belongs to the MAPEG family.

  • Post-translational
    modifications

    Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.

  • Cellular localization

    Microsome. Mitochondrion outer membrane. Endoplasmic reticulum membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • MGST1 antibody
    • Glutathione S transferase 12 antibody
    • GST12 antibody
    • MGST 1 antibody
    • MGST antibody
    • MGST I antibody
    • MGST1 antibody
    • MGST1_HUMAN antibody
    • Microsomal glutathione S-transferase 1 antibody
    • Microsomal GST 1 antibody
    • Microsomal GST-1 antibody
    • Microsomal GST-I antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] (ab131059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] (ab131059)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling MGST1 with Purified ab131059 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-MGST1 antibody [EPR7934] (ab131059)
    Western blot - Anti-MGST1 antibody [EPR7934] (ab131059)
    All lanes : Anti-MGST1 antibody [EPR7934] (ab131059) at 1/5000 dilution

    Lane 1 : Human fetal liver lysates
    Lane 2 : Mouse liver lysates
    Lane 3 : Rat liver lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 18 kDa



    Blocking and dilutuing buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-MGST1 antibody [EPR7934] (ab131059)
    Flow Cytometry - Anti-MGST1 antibody [EPR7934] (ab131059)

    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MGST1 with purified ab131059 at 1:40 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] (ab131059)
    Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] (ab131059)
    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Microsomal Glutathione S-transferase 2 with Purified ab131059 at 1:100 dilution. Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-MGST1 antibody [EPR7934] (ab131059)
    Western blot - Anti-MGST1 antibody [EPR7934] (ab131059)
    All lanes : Anti-MGST1 antibody [EPR7934] (ab131059) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : HepG2 lysate
    Lane 3 : HT-1376 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 18 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] (ab131059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] (ab131059)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labelling MGST1 with ab131059 antibody at 1/50 dilution.

  • OI-RD Scanning - Anti-MGST1 antibody [EPR7934] (ab131059)
    OI-RD Scanning - Anti-MGST1 antibody [EPR7934] (ab131059)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-MGST1 antibody [EPR7934] (ab131059)
    Anti-MGST1 antibody [EPR7934] (ab131059)

References (3)

Publishing research using ab131059? Please let us know so that we can cite the reference in this datasheet.

ab131059 has been referenced in 3 publications.

  • Kobayashi T  et al. Homeostatic Control of Sebaceous Glands by Innate Lymphoid Cells Regulates Commensal Bacteria Equilibrium. Cell 176:982-997.e16 (2019). PubMed: 30712873
  • Mao ZP  et al. Expression and significance of glucose transporter-1, P-glycoprotein, multidrug resistance-associated protein and glutathione S-transferase-p in laryngeal carcinoma. Oncol Lett 9:806-810 (2015). PubMed: 25621055
  • Walters MS  et al. Smoking accelerates aging of the small airway epithelium. Respir Res 15:94 (2014). IHC-P ; Human . PubMed: 25248511

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