RecombinantRabMAb

Recombinant Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7934] to MGST1 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MGST1 antibody [EPR7934] - BSA and Azide free
    See all MGST1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7934] to MGST1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human MGST1 aa 1-100.
    Database link: P10620

  • Positive control

    • IHC-P: Human liver tissue.

  • General notes

    ab232469 is the carrier-free version of ab131059 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232469 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Microsomal Glutathione S-transferase 1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab232469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse and rat.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.

  • Tissue specificity

    Highly expressed in liver.

  • Sequence similarities

    Belongs to the MAPEG family.

  • Post-translational
    modifications

    Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.

  • Cellular localization

    Microsome. Mitochondrion outer membrane. Endoplasmic reticulum membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • MGST1 antibody
    • Glutathione S transferase 12 antibody
    • GST12 antibody
    • MGST 1 antibody
    • MGST antibody
    • MGST I antibody
    • MGST1 antibody
    • MGST1_HUMAN antibody
    • Microsomal glutathione S-transferase 1 antibody
    • Microsomal GST 1 antibody
    • Microsomal GST-1 antibody
    • Microsomal GST-I antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labelling MGST1 with ab131059 antibody at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Flow Cytometry - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MGST1 with purified ab131059 at 1:40 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

  • Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Microsomal Glutathione S-transferase 2 with Purified ab131059 at 1:100 dilution. Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 µg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

  • Other - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Other - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling MGST1 with Purified ab131059 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab232469? Please let us know so that we can cite the reference in this datasheet.

ab232469 has not yet been referenced specifically in any publications.

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