Recombinant Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)](https://www.abcam.com/ps/products/232/ab232469/Images/ab232469-3-MicrosomalGlutathioneStransferase1Primaryantibodiesab1310592.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7934] to MGST1 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MGST1 antibody [EPR7934] - BSA and Azide free
See all MGST1 primary antibodies -
Description
Rabbit monoclonal [EPR7934] to MGST1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human liver tissue.
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General notes
ab232469 is the carrier-free version of ab131059.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7934 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
|
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
We do not guarantee IHC-P for mouse and rat. |
| Notes |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa). |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. We do not guarantee IHC-P for mouse and rat. |
Target
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity. -
Tissue specificity
Highly expressed in liver. -
Sequence similarities
Belongs to the MAPEG family. -
Post-translational
modificationsPeroxynitrite induces nitration at Tyr-93 which activates the enzyme. -
Cellular localization
Microsome. Mitochondrion outer membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 4257 Human
- Entrez Gene: 56615 Mouse
- Entrez Gene: 171341 Rat
- Omim: 138330 Human
- SwissProt: P10620 Human
- SwissProt: Q91VS7 Mouse
- SwissProt: P08011 Rat
- Unigene: 389700 Human
see all -
Alternative names
- MGST1 antibody
- Glutathione S transferase 12 antibody
- GST12 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
Immunohistochemical analysis of paraffin embedded Human liver tissue labelling MGST1 with ab131059 antibody at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)](https://www.abcam.com/ps/products/232/ab232469/Images/ab232469-5-ab1310596ab131059FC1.jpg)
Flow Cytometry (Intracellular) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MGST1with purified ab131059 at 1/40 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).
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![Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)](https://www.abcam.com/ps/products/232/ab232469/Images/ab232469-4-ab1310595ab131059ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Microsomal Glutathione S-transferase 2 with Purified ab131059 at 1:100 dilution. Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 µg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).
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![OI-RD Scanning - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)](https://www.abcam.com/ps/products/232/ab232469/Images/ab232469-2-MicrosomalGlutathioneStransferase1Primaryantibodiesab1310593.jpg)
OI-RD Scanning - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)](https://www.abcam.com/ps/products/232/ab232469/Images/ab232469-308867-059.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling MGST1 with Purified ab131059 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).
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![Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)](https://www.abcam.com/ps/products/232/ab232469/Images/ab232469-6-benefits-of-recombinant-antibodies.png)
Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab232469 has not yet been referenced specifically in any publications.
