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    mgst1-antibody-epr7934-bsa-and-azide-free-ab232469.pdf

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RecombinantRabMAb

Recombinant Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
  • Flow Cytometry (Intracellular) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
  • Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
  • OI-RD Scanning - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
  • Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7934] to MGST1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

You may also be interested in

Conjugation
Product image
PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
Protein
Product image
Recombinant Human MGST1 protein (ab114792)
Primary
Product image
Anti-MGST1 antibody [EPR7934] (ab131059)

View more associated products

Overview

  • Product name

    Anti-MGST1 antibody [EPR7934] - BSA and Azide free
    See all MGST1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7934] to MGST1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human liver tissue.
  • General notes

    ab232469 is the carrier-free version of ab131059.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7934
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Drugs and Chemicals
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Developmental Biology
    • Post embryonic development
    • Aging
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Associated products

  • Alternative Versions

    • Anti-MGST1 antibody [EPR7934] (ab131059)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human MGST1 protein (ab114792)
  • Related Products

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab232469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse and rat.

Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse and rat.

Target

  • Function

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.
  • Tissue specificity

    Highly expressed in liver.
  • Sequence similarities

    Belongs to the MAPEG family.
  • Post-translational
    modifications

    Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.
  • Cellular localization

    Microsome. Mitochondrion outer membrane. Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession P10620 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4257 Human
    • Entrez Gene: 56615 Mouse
    • Entrez Gene: 171341 Rat
    • Omim: 138330 Human
    • SwissProt: P10620 Human
    • SwissProt: Q91VS7 Mouse
    • SwissProt: P08011 Rat
    • Unigene: 389700 Human
    • Unigene: 14796 Mouse
    • Unigene: 2580 Rat
    see all
  • Alternative names

    • MGST1 antibody
    • Glutathione S transferase 12 antibody
    • GST12 antibody
    • MGST 1 antibody
    • MGST antibody
    • MGST I antibody
    • MGST1 antibody
    • MGST1_HUMAN antibody
    • Microsomal glutathione S-transferase 1 antibody
    • Microsomal GST 1 antibody
    • Microsomal GST-1 antibody
    • Microsomal GST-I antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labelling MGST1 with ab131059 antibody at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Flow Cytometry (Intracellular) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MGST1with purified ab131059 at 1/40 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059). 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Microsomal Glutathione S-transferase 2 with Purified ab131059 at 1:100 dilution. Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 µg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

  • OI-RD Scanning - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    OI-RD Scanning - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling MGST1 with Purified ab131059 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

  • Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab232469? Please let us know so that we can cite the reference in this datasheet.

ab232469 has not yet been referenced specifically in any publications.

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