Recombinant Anti-MGST1 antibody [EPR7935] (ab129175)
![Western blot - Anti-MGST1 antibody [EPR7935] (ab129175)](https://www.abcam.com/ps/products/129/ab129175/Images/ab129175-143196-anti-microsomal-glutathione-s-transferase-1-antibody-epr7935-western-blot.jpg "Western blot - Anti-MGST1 antibody [EPR7935] (ab129175)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7935] to MGST1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MGST1 antibody [EPR7935]
See all MGST1 primary antibodies -
Description
Rabbit monoclonal [EPR7935] to MGST1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human MGST1 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P10620 -
Positive control
- Fetal liver lysate, HepG2 cells and cell lysate, K562 cell lysate, HT 1376 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.40 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7935 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
|
|
| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa). |
|
ICC/IF
1/100 - 1/250. |
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.
Target
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity. -
Tissue specificity
Highly expressed in liver. -
Sequence similarities
Belongs to the MAPEG family. -
Post-translational
modificationsPeroxynitrite induces nitration at Tyr-93 which activates the enzyme. -
Cellular localization
Microsome. Mitochondrion outer membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 4257 Human
- Entrez Gene: 56615 Mouse
- Entrez Gene: 171341 Rat
- Omim: 138330 Human
- SwissProt: P10620 Human
- SwissProt: Q91VS7 Mouse
- SwissProt: P08011 Rat
- Unigene: 389700 Human
see all -
Alternative names
- MGST1 antibody
- Glutathione S transferase 12 antibody
- GST12 antibody
see all
Images
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Western blot - Anti-MGST1 antibody [EPR7935] (ab129175)All lanes : Anti-MGST1 antibody [EPR7935] (ab129175) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HT 1376 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody. at 1/2000 dilution
Predicted band size: 18 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7935] (ab129175)](https://www.abcam.com/ps/products/129/ab129175/Images/ab129175-143195-anti-microsomal-glutathione-s-transferase-1-antibody-epr7935-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7935] (ab129175)ab129175, at 1/100, staining MGST1 in HepG2 cells by Immunofluorescence.
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![OI-RD Scanning - Anti-MGST1 antibody [EPR7935] (ab129175)](https://www.abcam.com/ps/products/129/ab129175/Images/ab129175-143194-anti-microsomal-glutathione-s-transferase-1-antibody-epr7935.jpg)
OI-RD Scanning - Anti-MGST1 antibody [EPR7935] (ab129175) -
![Anti-MGST1 antibody [EPR7935] (ab129175)](https://www.abcam.com/ps/products/129/ab129175/Images/ab129175-4-benefits-of-recombinant-antibodies.png)
Anti-MGST1 antibody [EPR7935] (ab129175)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab129175 has not yet been referenced specifically in any publications.