Product nameAnti-MGST1 antibody [EPR7935]
See all MGST1 primary antibodies
DescriptionRabbit monoclonal [EPR7935] to MGST1
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human MGST1 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P10620
- Fetal liver lysate, HepG2 cells and cell lysate, K562 cell lysate, HT 1376 cell lysate.
This product was previously labelled as Microsomal Glutathione S-transferase 1
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.40 x 10 -12 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).|
|ICC/IF||1/100 - 1/250.|
FunctionConjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.
Tissue specificityHighly expressed in liver.
Sequence similaritiesBelongs to the MAPEG family.
modificationsPeroxynitrite induces nitration at Tyr-93 which activates the enzyme.
Cellular localizationMicrosome. Mitochondrion outer membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- MGST1 antibody
- Glutathione S transferase 12 antibody
- GST12 antibody
All lanes : Anti-MGST1 antibody [EPR7935] (ab129175) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HT 1376 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody. at 1/2000 dilution
Predicted band size: 18 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
ab129175, at 1/100, staining MGST1 in HepG2 cells by Immunofluorescence.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab129175 has not yet been referenced specifically in any publications.