Recombinant
RabMAb

Recombinant Anti-MGST1 antibody [EPR7935] (ab129175)

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Overview

  • Product name

    Anti-MGST1 antibody [EPR7935]
    See all MGST1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7935] to MGST1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human MGST1 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P10620

  • Positive control

    • Fetal liver lysate, HepG2 cells and cell lysate, K562 cell lysate, HT 1376 cell lysate.

  • General notes

     

     

     This product was previously labelled as Microsomal Glutathione S-transferase 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    • Function

      Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.

    • Tissue specificity

      Highly expressed in liver.

    • Sequence similarities

      Belongs to the MAPEG family.

    • Post-translational
      modifications

      Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.

    • Cellular localization

      Microsome. Mitochondrion outer membrane. Endoplasmic reticulum membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • MGST1 antibody
      • Glutathione S transferase 12 antibody
      • GST12 antibody
      • MGST 1 antibody
      • MGST antibody
      • MGST I antibody
      • MGST1 antibody
      • MGST1_HUMAN antibody
      • Microsomal glutathione S-transferase 1 antibody
      • Microsomal GST 1 antibody
      • Microsomal GST-1 antibody
      • Microsomal GST-I antibody
      see all

    Images

    • Western blot - Anti-MGST1 antibody [EPR7935] (ab129175)
      Western blot - Anti-MGST1 antibody [EPR7935] (ab129175)
      All lanes : Anti-MGST1 antibody [EPR7935] (ab129175) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : HT 1376 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody. at 1/2000 dilution

      Predicted band size: 18 kDa
      Observed band size: 16 kDa
      why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7935] (ab129175)
      Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7935] (ab129175)

      ab129175, at 1/100, staining MGST1 in HepG2 cells by Immunofluorescence.

    • Other - Anti-MGST1 antibody [EPR7935] (ab129175)
      Other - Anti-MGST1 antibody [EPR7935] (ab129175)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab129175 has not yet been referenced specifically in any publications.

    Publishing research using ab129175? Please let us know so that we can cite the reference in this datasheet.

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