Key features and details
- Mouse monoclonal [2G10CG7] to MGST3
- Suitable for: ICC/IF, IP
- Reacts with: Rat, Human
- Isotype: IgG2a
Product nameAnti-MGST3 antibody [2G10CG7]
See all MGST3 primary antibodies
DescriptionMouse monoclonal [2G10CG7] to MGST3
Tested applicationsSuitable for: ICC/IF, IPmore details
Species reactivityReacts with: Rat, Human
This information is considered to be commercially sensitive.
- ICC/IF: HDFn cells IP: Human and Rat liver lysates
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
Purification notesThe purity of ab110309 is near homogeneity as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Light chain typekappa
Our Abpromise guarantee covers the use of ab110309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 2 µg/ml.|
|IP||Use at an assay dependent concentration.|
FunctionAlso functions as a glutathione peroxidase.
Tissue specificityPredominantly expressed in heart, skeletal muscle, and adrenal cortex. Also found in brain, placenta, liver, kidney, pancreas, thyroid, testis and ovary. Almost absent in lung, thymus and peripheral blood leukocytes.
Sequence similaritiesBelongs to the MAPEG family.
Cellular localizationEndoplasmic reticulum membrane. Microsome membrane. Microsome membrane.
- Information by UniProt
- Mgst3 antibody
- MGST3_HUMAN antibody
- Microsomal glutathione S transferase 3 antibody
Immunocytochemistry image of ab110309-stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with ab110309 at 2 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. Target protein locates primarily in the mitochondria.
Using MitoSciences' standard immunoprecipitation protocol, ab110309 pulls down the 15kDa MGST3 protein from human and rat liver samples. HepG2 lysate and mouse liver lysate yielded smaller amounts. This identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.
ab110309 has not yet been referenced specifically in any publications.