Recombinant Anti-MGST3 antibody [EPR12352] (ab192254)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12352] to MGST3
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MGST3 antibody [EPR12352]
See all MGST3 primary antibodies -
Description
Rabbit monoclonal [EPR12352] to MGST3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A673, HepG2, Human fetal brain, and mouse spleen lysates; Human and mouse cardiac muscle and rat liver tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12352 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/20.
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WB |
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
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Notes |
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/20. |
WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). |
Target
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Function
Also functions as a glutathione peroxidase. -
Tissue specificity
Predominantly expressed in heart, skeletal muscle, and adrenal cortex. Also found in brain, placenta, liver, kidney, pancreas, thyroid, testis and ovary. Almost absent in lung, thymus and peripheral blood leukocytes. -
Sequence similarities
Belongs to the MAPEG family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 4259 Human
- Entrez Gene: 66447 Mouse
- Entrez Gene: 289197 Rat
- Omim: 604564 Human
- SwissProt: O14880 Human
- SwissProt: Q9CPU4 Mouse
- Unigene: 191734 Human
- Unigene: 218286 Mouse
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Alternative names
- Mgst3 antibody
- MGST3_HUMAN antibody
- Microsomal glutathione S transferase 3 antibody
see all
Images
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All lanes : Anti-MGST3 antibody [EPR12352] (ab192254) at 1/10000 dilution
Lane 1 : A673 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa -
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
(inset: negative control).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-MGST3 antibody [EPR12352] (ab192254) at 1/1000 dilution + Human fetal brain tissue lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 17 kDa -
Anti-MGST3 antibody [EPR12352] (ab192254) at 1/1000 dilution + mouse spleen tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa -
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
(inset: negative control).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
(inset: negative control).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab192254 at 1/20 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab192254 has been referenced in 1 publication.
- Liu X et al. The metabolic genomic atlas reveals potential drivers and clinically relevant insights into the etiology of esophageal squamous cell carcinoma. Theranostics 12:6160-6178 (2022). PubMed: 36168622