Overview

  • Product name

    Anti-mH2A1 antibody [EPR9358]
    See all mH2A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9358] to mH2A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human mH2A1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Fetal kidney, HepG2, HeLa, and MCF7 cell lysates, Human cervical carcinoma and Human colon tissue.
  • General notes

     

     

    Previously labelled as macroH2A.1.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9358
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Contains 1 histone H2A domain.
      Contains 1 Macro domain.
    • Post-translational
      modifications

      Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin.
    • Cellular localization

      Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.
    • Information by UniProt
    • Database links

    • Alternative names

      • Core histone macro h2a.1 antibody
      • Core histone macro-H2A.1 antibody
      • H2A histone family member Y antibody
      • H2A.y antibody
      • H2A/y antibody
      • H2AF12M antibody
      • H2AFJ antibody
      • H2afy antibody
      • H2AY_HUMAN antibody
      • Histone H2A.Y antibody
      • Histone macroH2A1 antibody
      • Histone macroH2A1.1 antibody
      • Histone macroH2A1.2 antibody
      • Macroh2a1 antibody
      • MACROH2A1.1 antibody
      • MacroH2A1.2 antibody
      • Medulloblastoma antigen MU MB 50.205 antibody
      • Medulloblastoma antigen MU-MB-50.205 antibody
      • mH2a antibody
      • mH2A1 antibody
      see all

    Images

    • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: mH2A1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: Hepg2 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab137117 observed at 40 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab137117 was shown to recognize mH2A1 when mH2A1 knockout samples were used, along with additional cross-reactive bands. Wild-type and mH2A1 knockout samples were subjected to SDS-PAGE. Ab137117 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver from wild-type and mH2A1/2 knock out tissue sections labeling mH2A1 with ab137117 at 1/400 dilution. Sections were fixed in formaldehyde; heat mediated antigen retivial was performed using a citrate buffer pH 6. An undiluted polyclonal horse anti-rabbit IgG (HRP-conjugated) was used as the secondary antibody.

      See Abreview

    • All lanes : Anti-mH2A1 antibody [EPR9358] (ab137117) at 1/1000 dilution

      Lane 1 : fetal kidney cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 40 kDa

    • Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labelling mH2A1 with ab137117 antibody at a dilution of 1/100.

    • Immunohistochemical analysis of paraffin embedded Human colon tissue labelling mH2A1 with ab137117 antibody at a dilution of 1/100.

    References

    This product has been referenced in:

    • Lo Re O  et al. Induction of cancer cell stemness by depletion of macrohistone H2A1 in hepatocellular carcinoma. Hepatology N/A:N/A (2017). Human . Read more (PubMed: 28913935) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver from WT and macroH2A1/2 KO)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6
    Permeabilization
    No
    Specification
    Liver from WT and macroH2A1/2 KO
    Blocking step
    PBS, 2% horse serum, 1% BSA, 0.1% Triton X-100, 0.05% Tween 20, 0.05% sodium azide as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative
    Formaldehyde

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    Verified customer

    Submitted Nov 16 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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