Anti-mH2A2 antibody (ab154687)
Key features and details
- Rabbit polyclonal to mH2A2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-mH2A2 antibody
See all mH2A2 primary antibodies -
Description
Rabbit polyclonal to mH2A2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Recombinant fragment corresponding to Human mH2A2 aa 1-197.
Database link: Q9P0M6 -
Positive control
- A431 and mouse brain whole cell lysate.
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General notes
Keep as concentrated solution.Previously labelled as Macro H2A.2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Mouse
Human
|
| All applications |
Cow
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa. |
Target
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Function
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation. -
Sequence similarities
Contains 1 histone H2A domain.
Contains 1 Macro domain. -
Cellular localization
Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. - Information by UniProt
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Database links
- Entrez Gene: 537167 Cow
- Entrez Gene: 55506 Human
- Entrez Gene: 404634 Mouse
- SwissProt: Q9P0M6 Human
- SwissProt: Q8CCK0 Mouse
- Unigene: 499953 Human
- Unigene: 272870 Mouse
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Alternative names
- Core histone macro H2A2.2 antibody
- Core histone macro-H2A.2 antibody
- Core histone macroH2A2.2 antibody
see all
Images
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Western blot - Anti-mH2A2 antibody (ab154687)Anti-mH2A2 antibody (ab154687) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 40 kDa
10% SDS PAGE -
Western blot - Anti-mH2A2 antibody (ab154687)Anti-mH2A2 antibody (ab154687) at 1/500 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 40 kDa
10% SDS PAGE
References (0)
ab154687 has not yet been referenced specifically in any publications.
