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    mh2a2-antibody-ab154687.pdf

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Epigenetics and Nuclear Signaling Histones Variants
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Anti-mH2A2 antibody (ab154687)

  • Datasheet
  • SDS
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Western blot - Anti-mH2A2 antibody (ab154687)
  • Western blot - Anti-mH2A2 antibody (ab154687)

Key features and details

  • Rabbit polyclonal to mH2A2
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Protein
Product image
Recombinant Human mH2A2 protein (ab131967)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-mH2A2 antibody
    See all mH2A2 primary antibodies
  • Description

    Rabbit polyclonal to mH2A2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human mH2A2 aa 1-197.
    Database link: Q9P0M6

  • Positive control

    • A431 and mouse brain whole cell lysate.
  • General notes

    Keep as concentrated solution.

    Previously labelled as Macro H2A.2.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • Variants

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human mH2A2 protein (ab131967)
  • Related Products

    • Recombinant Human mH2A2 protein (ab131967)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Human
All applications
Cow
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa.

Target

  • Function

    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
  • Sequence similarities

    Contains 1 histone H2A domain.
    Contains 1 Macro domain.
  • Cellular localization

    Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.
  • Target information above from: UniProt accession Q9P0M6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 537167 Cow
    • Entrez Gene: 55506 Human
    • Entrez Gene: 404634 Mouse
    • SwissProt: Q9P0M6 Human
    • SwissProt: Q8CCK0 Mouse
    • Unigene: 499953 Human
    • Unigene: 272870 Mouse
    • Alternative names

      • Core histone macro H2A2.2 antibody
      • Core histone macro-H2A.2 antibody
      • Core histone macroH2A2.2 antibody
      • H2A histone family member Y2 antibody
      • H2AFY2 antibody
      • H2AW_HUMAN antibody
      • Histone macroH2A2 antibody
      • Macro H2A.2 antibody
      • Macro H2A2 antibody
      • MacroH2A.2 antibody
      • MacroH2A2 antibody
      • mH2A2 antibody
      see all

    Images

    • Western blot - Anti-mH2A2 antibody (ab154687)
      Western blot - Anti-mH2A2 antibody (ab154687)
      Anti-mH2A2 antibody (ab154687) at 1/1000 dilution + A431 whole cell lysate at 30 µg

      Predicted band size: 40 kDa



      10% SDS PAGE
    • Western blot - Anti-mH2A2 antibody (ab154687)
      Western blot - Anti-mH2A2 antibody (ab154687)
      Anti-mH2A2 antibody (ab154687) at 1/500 dilution + Mouse brain whole cell lysate at 50 µg

      Predicted band size: 40 kDa



      10% SDS PAGE

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab154687? Please let us know so that we can cite the reference in this datasheet.

    ab154687 has not yet been referenced specifically in any publications.

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