Anti-mH2A2 antibody (ab246995)
Key features and details
- Rabbit polyclonal to mH2A2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-mH2A2 antibody
See all mH2A2 primary antibodies -
Description
Rabbit polyclonal to mH2A2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human mH2A2 aa 150-300.
Database link: Q9P0M6 -
Positive control
- IHC-P: Human cerebellum, endometrium and testis tissue. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab246995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
Notes |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Function
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation. -
Sequence similarities
Contains 1 histone H2A domain.
Contains 1 Macro domain. -
Cellular localization
Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. - Information by UniProt
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Database links
- Entrez Gene: 55506 Human
- SwissProt: Q9P0M6 Human
- Unigene: 499953 Human
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Alternative names
- Core histone macro H2A2.2 antibody
- Core histone macro-H2A.2 antibody
- Core histone macroH2A2.2 antibody
see all
Images
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PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for mH2A2 (green) using ab246995 at 4 μg/ml in ICC/IF.
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Formalin-fixed, paraffin-embedded human cerebellum tissue stained for mH2A2 with ab246995 at a 1/500 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human testis tissue stained for mH2A2 with ab246995 at a 1/500 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human endometrium tissue stained for mH2A2 with ab246995 at a 1/500 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human liver tissue shows absence of immunoreactivity for mH2A2 when incubated with ab246995 at a 1/500 dilution (negative control).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab246995 has not yet been referenced specifically in any publications.