Key features and details
- Rabbit polyclonal to mH2A2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-mH2A2 antibody
See all mH2A2 primary antibodies
DescriptionRabbit polyclonal to mH2A2
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human cerebellum, endometrium and testis tissue. ICC/IF: U-2 OS cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab246995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
FunctionVariant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
Sequence similaritiesContains 1 histone H2A domain.
Contains 1 Macro domain.
Cellular localizationNucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.
- Information by UniProt
- Core histone macro H2A2.2 antibody
- Core histone macro-H2A.2 antibody
- Core histone macroH2A2.2 antibody
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for mH2A2 (green) using ab246995 at 4 μg/ml in ICC/IF.
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for mH2A2 with ab246995 at a 1/500 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human testis tissue stained for mH2A2 with ab246995 at a 1/500 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human endometrium tissue stained for mH2A2 with ab246995 at a 1/500 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human liver tissue shows absence of immunoreactivity for mH2A2 when incubated with ab246995 at a 1/500 dilution (negative control).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246995 has not yet been referenced specifically in any publications.