Product nameAnti-mH2A2 antibody
See all mH2A2 primary antibodies
DescriptionRabbit polyclonal to mH2A2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human mH2A2 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following cell lysates: HeLa nuclear extract.
Previously labelled as Macro H2A.2.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab4173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 40 kDa).|
FunctionVariant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
Sequence similaritiesContains 1 histone H2A domain.
Contains 1 Macro domain.
Cellular localizationNucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.
- Information by UniProt
- Core histone macro H2A2.2 antibody
- Core histone macro-H2A.2 antibody
- Core histone macroH2A2.2 antibody
Lane 1 : 2 ug of chicken erythrocyte chromatin fraction stained with ponceau.
Lane 2 : 2 ug of chicken liver chromatin fraction stained with ponceau.
Lanes 3-4 : Anti-mH2A2 antibody (ab4173) at 1/500 dilution
Lane 3 : 2 ug of chicken erythrocyte chromatin fraction.
Lane 4 : 2 ug of chicken liver chromatin fraction.
Lanes 3-4 : Secondary antibody used at a dilution of 1/3000.
Performed under reducing conditions.
Predicted band size: 40 kDa
CL = chicken liver and CE= chicken erythrocyte. Approximately 2 micrograms were loaded in each lane, blotted and reacted with primary antibody at dilution of 1:500 and 1:3000 for the secondary.
Previously, the problem has been that mH2A2 is not very ubiquitous compared to macro H2A.1. While this makes it good for commercialization purposes it is hard to prepare chromatin fractions that are enriched in this fraction. However, these images show a ponceau stain (left hand side blot) and the western using the ab4173 antibody. There is one specific band.
All lanes : Anti-mH2A2 antibody (ab4173) at 1 µg/ml
Lane 1 : HeLa nuclear extract
Lane 2 : HeLa nuclear extract with Human Macro H2A.2 peptide (ab15023) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size: 40 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
ab4173 specifically recognises mH2A2 at approximately 50 kDa (lane1), which can be blocked using the immunizing peptide (lane2). mH2A2 is known to migrate slower than its predicted molecular weight of 40 kDa (PMID: 1529340).
This product has been referenced in:
- Yildirim O et al. A System for Genome-Wide Histone Variant Dynamics In ES Cells Reveals Dynamic MacroH2A2 Replacement at Promoters. PLoS Genet 10:e1004515 (2014). CHIPseq ; Mouse . Read more (PubMed: 25102063) »
- Boulard M et al. Histone variant macroH2A1 deletion in mice causes female-specific steatosis. Epigenetics Chromatin 3:8 (2010). WB ; Mouse . Read more (PubMed: 20359320) »