Overview

  • Product name
  • Description
    Rabbit polyclonal to mH2A2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human mH2A2 aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab15023)

  • Positive control
    • This antibody gave a positive signal in the following cell lysates: HeLa nuclear extract.
  • General notes

    Previously labelled as Macro H2A.2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 40 kDa). Can be blocked with Macro H2A.2 peptide (ab15023).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
    • Sequence similarities
      Contains 1 histone H2A domain.
      Contains 1 Macro domain.
    • Cellular localization
      Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.
    • Information by UniProt
    • Database links
    • Alternative names
      • Core histone macro H2A2.2 antibody
      • Core histone macro-H2A.2 antibody
      • Core histone macroH2A2.2 antibody
      • H2A histone family member Y2 antibody
      • H2AFY2 antibody
      • H2AW_HUMAN antibody
      • Histone macroH2A2 antibody
      • Macro H2A.2 antibody
      • Macro H2A2 antibody
      • MacroH2A.2 antibody
      • MacroH2A2 antibody
      • mH2A2 antibody
      see all

    Images

    • Lane 1 : 2 ug of chicken erythrocyte chromatin fraction stained with ponceau.
      Lane 2 : 2 ug of chicken liver chromatin fraction stained with ponceau.
      Lanes 3-4 : Anti-mH2A2 antibody (ab4173) at 1/500 dilution

      Lane 3 : 2 ug of chicken erythrocyte chromatin fraction.
      Lane 4 : 2 ug of chicken liver chromatin fraction.

      Secondary
      Lanes 3-4 : Secondary antibody used at a dilution of 1/3000.

      Performed under reducing conditions.

      Predicted band size: 40 kDa



      CL = chicken liver and CE= chicken erythrocyte. Approximately 2 micrograms were loaded in each lane, blotted and reacted with primary antibody at dilution of 1:500 and 1:3000 for the secondary.

      Previously, the problem has been that mH2A2 is not very ubiquitous compared to macro H2A.1. While this makes it good for commercialization purposes it is hard to prepare chromatin fractions that are enriched in this fraction. However, these images show a ponceau stain (left hand side blot) and the western using the ab4173 antibody. There is one specific band.

    • All lanes : Anti-mH2A2 antibody (ab4173) at 1 µg/ml

      Lane 1 : HeLa nuclear extract
      Lane 2 : HeLa nuclear extract with Human Macro H2A.2 peptide (ab15023) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 40 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?



      ab4173 specifically recognises mH2A2 at approximately 50 kDa (lane1), which can be blocked using the immunizing peptide (lane2). mH2A2 is known to migrate slower than its predicted molecular weight of 40 kDa (PMID: 1529340).

    References

    This product has been referenced in:
    • Yildirim O  et al. A System for Genome-Wide Histone Variant Dynamics In ES Cells Reveals Dynamic MacroH2A2 Replacement at Promoters. PLoS Genet 10:e1004515 (2014). CHIPseq ; Mouse . Read more (PubMed: 25102063) »
    • Boulard M  et al. Histone variant macroH2A1 deletion in mice causes female-specific steatosis. Epigenetics Chromatin 3:8 (2010). WB ; Mouse . Read more (PubMed: 20359320) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Question
    Answer

    I am very pleased to hear you would like to accept our offer and test ab4173 & ab102126 in mouse samples. This code will be worth the price you pay for ab4173 & ab102126 and can be used with any single future primary antibody purchase before the expiration date. To redeem this offer, you must first submit your Abreview with your mouse data. Please include this code in the “Additional Comments” section so that we will know the review is for this promotion. The code will be active once the Abreview has been submitted. The code can be redeemed in the following ways: 1) Call to place your order by phone and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and remember to enter your promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up