Anti-mH2A2 antibody [CL5263] (ab242357)

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Overview

  • Product name

    Anti-mH2A2 antibody [CL5263]
    See all mH2A2 primary antibodies

  • Description

    Mouse monoclonal [CL5263] to mH2A2

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide within Human mH2A2 aa 133-147. The exact sequence is proprietary.
    Sequence:

    EKRGRKATSGKKGGK


    Database link: Q9P0M6

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human testis, fallopian tube, cerebral cortex and kidney tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    CL5263

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab242357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.

  • Sequence similarities

    Contains 1 histone H2A domain.
    Contains 1 Macro domain.

  • Cellular localization

    Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.
  • Information by UniProt

  • Database links

    • Alternative names

      • Core histone macro H2A2.2 antibody
      • Core histone macro-H2A.2 antibody
      • Core histone macroH2A2.2 antibody
      • H2A histone family member Y2 antibody
      • H2AFY2 antibody
      • H2AW_HUMAN antibody
      • Histone macroH2A2 antibody
      • Macro H2A.2 antibody
      • Macro H2A2 antibody
      • MacroH2A.2 antibody
      • MacroH2A2 antibody
      • mH2A2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody [CL5263] (ab242357)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody [CL5263] (ab242357)

      Paraffin-embedded human testis tissue stained for mH2A2 with ab242357 at 1/200 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody [CL5263] (ab242357)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody [CL5263] (ab242357)

      Paraffin-embedded human fallopian tube tissue stained for mH2A2 with ab242357 at 1/200 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody [CL5263] (ab242357)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody [CL5263] (ab242357)

      Paraffin-embedded human cerebral cortex tissue stained for mH2A2 with ab242357 at 1/200 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody [CL5263] (ab242357)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody [CL5263] (ab242357)

      Paraffin-embedded human kidney tissue stained for mH2A2 with ab242357 at 1/200 dilution in immunohistochemical analysis.

    References

    ab242357 has not yet been referenced specifically in any publications.

    Publishing research using ab242357? Please let us know so that we can cite the reference in this datasheet.

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