Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (ab25244)
Key features and details
- Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db/H2-D1
- Suitable for: IHC-Fr, Functional Studies, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13]
See all MHC Class 1 H2 Db/H2-D1 primary antibodies -
Description
Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db/H2-D1 -
Host species
Mouse -
Specificity
ab25244 recognizes the a3 domain of H2Db class I MHC antigen. The antibody cross-reacts with the a3 domain of H2Ld, H2Dq and H2Lq, but not H2Kd or H2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed. -
Tested applications
Suitable for: IHC-Fr, Functional Studies, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse MHC Class 1 H2 Db/H2-D1. BDF1 mouse splenocytes
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Epitope
Recognizes an epitope on the a chain of H2Db. -
General notes
ab25244 can be used for complement-dependent cytoxicity assays.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading...
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Primary antibody notes
ab25244 can be used for complement-dependent cytoxicity assays. -
Clonality
Monoclonal -
Clone number
27-11-13 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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Functional Studies |
Use at an assay dependent concentration.
Complement-dependent cytoxicity assay. |
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Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-Fr
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. Complement-dependent cytoxicity assay. |
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells. -
Cellular localization
Cell Membrane; single pass type I membrane protein -
Database links
- Entrez Gene: 14964 Mouse
- SwissProt: P01899 Mouse
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Alternative names
- H 2D cell surface glycoprotein antibody
- H2 class I histocompatibility antigen, DB alpha chain antibody
- H2D(B) antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab25244 has been referenced in 2 publications.
- Walsh SR et al. Type I IFN blockade uncouples immunotherapy-induced antitumor immunity and autoimmune toxicity. J Clin Invest 129:518-530 (2019). PubMed: 30422820
- Dower SK et al. The interaction of monoclonal antibodies with MHC class I antigens on mouse spleen cells. I. Analysis of the mechanism of binding. J Immunol 132:751-8 (1984). PubMed: 6690617