Anti-MHC Class 1 H2 Db antibody [27-11-13] (FITC) (ab25028)

Overview

  • Product name
    Anti-MHC Class 1 H2 Db antibody [27-11-13] (FITC)
    See all MHC Class 1 H2 Db primary antibodies
  • Description
    Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db (FITC)
  • Host species
    Mouse
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    ab25028 recognizes with the a3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the a3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse C3H.SW splenocytes

  • Epitope
    An epitope on the a chain of H-2Db

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Affinity purified
  • Clonality
    Monoclonal
  • Clone number
    27-11-13
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab18455 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. (Complement-dependent cytoxicity assay)

Target

  • Relevance
    In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
  • Cellular localization
    Cell Membrane; single pass type I membrane protein
  • Database links
  • Alternative names
    • H 2D cell surface glycoprotein antibody
    • H2 class I histocompatibility antigen, DB alpha chain antibody
    • H2D(B) antibody
    • H2D1 antibody
    • Histocompatibility 2, D region locus 1 antibody
    see all

References

ab25028 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Both ab21321 and ab25028 recognize extracellular epitopes and can be used to stain live cells for FACS.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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