• Product name
    Anti-MHC Class 1 H2 Db antibody [28-14-8]
    See all MHC Class 1 H2 Db primary antibodies
  • Description
    Mouse monoclonal [28-14-8] to MHC Class 1 H2 Db
  • Host species
  • Specificity
    ab25228 binds to the alpha3 domain of H2Db in the presence or absence of beta2 microglobulin. It cross reacts with the alpha3 domain of H2Ld but not Kd or Dd, and with H-Dq and/or Lq.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    C3H.SW splenocytes (Mouse)

  • Positive control
    • IHC-P: Mouse spleen FFPE tissue sections.
  • General notes

    ab25228 has been shown to block H2Ld-specific and H2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, but it does not block recognition of H2Ld-positive target cells by Ly-6G2-positive NK cells.



  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • Purity
  • Purification notes
    Purified from ascites.
  • Primary antibody notes
    ab25228 has been shown to block H2Ld-specific and H2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, but it does not block recognition of H2Ld-positive target cells by Ly-6G2-positive NK cells.
  • Clonality
  • Clone number
  • Isotype
  • Light chain type
  • Research areas


Our Abpromise guarantee covers the use of ab25228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


IHC-Fr Use a concentration of 2 µg/ml.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Relevance
    In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
  • Cellular localization
    Cell Membrane; single pass type I membrane protein
  • Database links
  • Alternative names
    • H 2D cell surface glycoprotein antibody
    • H2 class I histocompatibility antigen, DB alpha chain antibody
    • H2D(B) antibody
    • H2D1 antibody
    • Histocompatibility 2, D region locus 1 antibody
    see all


  • Flow Cytometry analysis of BALB/c mouse splenocytes labeling MHC Class 1 H2 Db with ab25228 at 1 μg/106 cells (purple). A Goat Anti-Mouse IgG2a, Human ads-FITC was used as the secondary antibody. Grey - Isotype Control, Mouse IgG2a-UNLB  followed by Goat Anti-Mouse IgG2a, Human ads-FITC.

  • Overlay histogram showing SP0/2 cells stained with ab25228 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25228, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • IHC image of MHC Class 1 H2 Db staining in mouse spleen formalin fixed paraffin embedded tissue section, using Mouse-on-Mouse detection kit, ab127055. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. The section was incubated with ab25228, 10µg/ml, for 15 mins at room temperature. The section was counterstained with haematoxylin and mounted with DPX.


This product has been referenced in:
  • Ogawa K  et al. Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors. Sci Rep 8:3615 (2018). Read more (PubMed: 29483626) »
  • Seo H  et al. IL-21-mediated reversal of NK cell exhaustion facilitates anti-tumour immunity in MHC class I-deficient tumours. Nat Commun 8:15776 (2017). IHC-P ; Human . Read more (PubMed: 28585539) »
See all 8 Publications for this product

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