Key features and details
- Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db/H2-D1 (FITC)
- Suitable for: Flow Cyt, IHC-Fr, Functional Studies
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Product nameAnti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (FITC)
See all MHC Class 1 H2 Db/H2-D1 primary antibodies
DescriptionMouse monoclonal [27-11-13] to MHC Class 1 H2 Db/H2-D1 (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
ab25028 recognizes with the a3 domain of H-2Db class I MHC (H2-D1) antigen. The antibody cross-reacts with the a3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.
Tested applicationsSuitable for: Flow Cyt, IHC-Fr, Functional Studiesmore details
Species reactivityReacts with: Mouse
Tissue, cells or virus corresponding to Mouse MHC Class 1 H2 Db/H2-D1.
EpitopeAn epitope on the a chain of H-2Db
- Flow cyt: Mouse C57BL/6 splenocytes.
This product was previously labelled as MHC Class 1 H2 Db
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
Light chain typekappa
Our Abpromise guarantee covers the use of ab25028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab18455 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration. (Complement-dependent cytoxicity assay)|
RelevanceIn the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
Cellular localizationCell Membrane; single pass type I membrane protein
- H 2D cell surface glycoprotein antibody
- H2 class I histocompatibility antigen, DB alpha chain antibody
- H2D(B) antibody
ab25028 has not yet been referenced specifically in any publications.