Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (Phycoerythrin) (ab25547)

Overview

  • Product name

    Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (Phycoerythrin)
    See all MHC Class 1 H2 Db/H2-D1 primary antibodies
  • Description

    Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db/H2-D1 (Phycoerythrin)
  • Host species

    Mouse
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity

    ab25547 recognizes the alpha3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the alpha3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.
  • Tested applications

    Suitable for: Functional Studies, Flow Cyt, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse MHC Class 1 H2 Db/H2-D1.

  • Epitope

    This antibody reacts with an epitope on the alpha chain [alpha3 domain] of H2Db.
  • Positive control

    • Flow cyt: Mouse BALB/c splenocytes.
  • General notes

     This product was previously labelled as MHC Class 1 H2 Db

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 16% Sucrose

    Also contains a stabilizing agent.
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    27-11-13
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution. (Complement-dependent cytoxicity assay)
Flow Cyt Use 0.2µg for 106 cells.
IHC-Fr Use at an assay dependent dilution. Acetone fixed only.

Target

  • Relevance

    In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
  • Cellular localization

    Cell Membrane; single pass type I membrane protein
  • Database links

  • Alternative names

    • H 2D cell surface glycoprotein antibody
    • H2 class I histocompatibility antigen, DB alpha chain antibody
    • H2D(B) antibody
    • H2D1 antibody
    • Histocompatibility 2, D region locus 1 antibody
    see all

Images

  • Flow cytometric analysis of mouse BALB/c mouse splenocytes stained for MHC Class 1 H2 Db/H2-D1 using ab25547 at 0.2 μg/106 cells.

References

This product has been referenced in:

  • Hoerter JA  et al. Coreceptor affinity for MHC defines peptide specificity requirements for TCR interaction with coagonist peptide-MHC. J Exp Med 210:1807-21 (2013). Read more (PubMed: 23940257) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry. We do not have a protocol specifically for acetone fixation. However, may I recommend the excellent resource at IHC world. The following link details technical tips to consider when performing acetone fixation. http://www.ihcworld.com/_technical_tips/crystat_section_ihc.htm Both ab25547 and ab25588 are directly conjugated with phycoerythrin and therefore will not require a secondary antibody to detect them. You will not require ab8649. Yes, you are correct you will only need to perform the primary antibody incubation i.e. Omit steps 9-14 from the ABC Immunohistochemical Staining protocol located at the following link. https://www.abcam.com/index.html?pageconfig=view_protocol&pid=158 Phycoerythrin or PE is excited by a similar wavelength to FITC (488nm) but emits at 578nm as opposed to 519nm (FITC). Therefore Im not certain you would see much emission using a FITC filter. It may be necessary to modify the detection wavelength if possible to detect a wavelength of 578nm. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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