Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (Phycoerythrin) (ab25547)


  • Product name

    Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (Phycoerythrin)
    See all MHC Class 1 H2 Db/H2-D1 primary antibodies
  • Description

    Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db/H2-D1 (Phycoerythrin)
  • Host species

  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity

    ab25547 recognizes the alpha3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the alpha3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.
  • Tested applications

    Suitable for: Functional Studies, Flow Cyt, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse MHC Class 1 H2 Db/H2-D1.

  • Epitope

    This antibody reacts with an epitope on the alpha chain [alpha3 domain] of H2Db.
  • Positive control

    • Flow cyt: Mouse BALB/c splenocytes.
  • General notes

     This product was previously labelled as MHC Class 1 H2 Db



  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 16% Sucrose

    Also contains a stabilizing agent.
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

  • Clone number

  • Isotype

  • Light chain type

  • Research areas


Our Abpromise guarantee covers the use of ab25547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution. (Complement-dependent cytoxicity assay)
Flow Cyt Use 0.2µg for 106 cells.
IHC-Fr Use at an assay dependent dilution. Acetone fixed only.


  • Relevance

    In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
  • Cellular localization

    Cell Membrane; single pass type I membrane protein
  • Database links

  • Alternative names

    • H 2D cell surface glycoprotein antibody
    • H2 class I histocompatibility antigen, DB alpha chain antibody
    • H2D(B) antibody
    • H2D1 antibody
    • Histocompatibility 2, D region locus 1 antibody
    see all


  • Flow cytometric analysis of mouse BALB/c mouse splenocytes stained for MHC Class 1 H2 Db/H2-D1 using ab25547 at 0.2 μg/106 cells.


This product has been referenced in:

  • Hoerter JA  et al. Coreceptor affinity for MHC defines peptide specificity requirements for TCR interaction with coagonist peptide-MHC. J Exp Med 210:1807-21 (2013). Read more (PubMed: 23940257) »
See 1 Publication for this product

Customer reviews and Q&As


Thank you for your enquiry. We do not have a protocol specifically for acetone fixation. However, may I recommend the excellent resource at IHC world. The following link details technical tips to consider when performing acetone fixation. Both ab25547 and ab25588 are directly conjugated with phycoerythrin and therefore will not require a secondary antibody to detect them. You will not require ab8649. Yes, you are correct you will only need to perform the primary antibody incubation i.e. Omit steps 9-14 from the ABC Immunohistochemical Staining protocol located at the following link. Phycoerythrin or PE is excited by a similar wavelength to FITC (488nm) but emits at 578nm as opposed to 519nm (FITC). Therefore Im not certain you would see much emission using a FITC filter. It may be necessary to modify the detection wavelength if possible to detect a wavelength of 578nm. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

For licensing inquiries, please contact

Sign up