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RecombinantRabMAb

Recombinant Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)

Reviews (3)Q&A (1)References (11)
Flow Cytometry - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)
  • Western blot - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)
  • Immunocytochemistry/ Immunofluorescence - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)
  • Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1394Y] to MHC class I + HLA A + HLA B
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

Overview

  • Product name

    Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y]
    See all MHC class I+HLA A+HLA B primary antibodies

  • Description

    Rabbit monoclonal [EPR1394Y] to MHC class I + HLA A + HLA B

  • Host species

    Rabbit

  • Specificity

    In our hands the MHC class I + HLA A + HLA B antibody (ab134189) did not work in WB with mouse samples, but customer data suggest that reactivity in ICC/IF and IHC. The target shows high sequence homology with HLA-C and HLA-E, and it is possible that this antibody will detect these proteins too.

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human MHC class I. The exact sequence is proprietary.

  • Positive control

    • WB: Raji, THP-1, and U-937 whole cell lysates. Human liver tissue lysate; ICC/IF: Raji cells; IHC-P: Human colon carcinoma tissue; Flow Cyt: Raji cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR1394Y

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

For unpurified use at 1/1000 - 1/10000.
IHC-P (2)
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.
Flow Cyt
1/100.

For unpurified use at 1/20 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/500.
Notes
WB
1/2000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

For unpurified use at 1/1000 - 1/10000.
IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.
Flow Cyt
1/100.

For unpurified use at 1/20 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/500.

Target

Images

  • Flow Cytometry - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)
    Flow Cytometry - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)

    Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells, labeling MHC class I+HLA A+HLA B with Purified ab134189 at 1:100 dilution (10 µg/ml) (Red). Cells were fixed with / and permeabilised with /. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling MHC class I+HLA A+HLA B with purified ab134189 at 1/4000 dilution (0.26 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)
    Western blot - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)
    All lanes : Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189) at 1/2000 dilution (Purified)

    Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 2 : Human liver lysate
    Lane 3 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
    Lane 4 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 41 kDa
    Observed band size: 44 kDa why is the actual band size different from the predicted?
    Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.



    Blocking/Diluting buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)
    Immunocytochemistry/ Immunofluorescence - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)

    Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling MHC class I+HLA A+HLA B with purified ab134189 at 1/500 dilution (4.3 µg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.

  • Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)
    Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)

References (11)

Publishing research using ab134189? Please let us know so that we can cite the reference in this datasheet.

ab134189 has been referenced in 11 publications.

  • Yao H  et al. A peptidic inhibitor for PD-1 palmitoylation targets its expression and functions. RSC Chem Biol 2:192-205 (2021). PubMed: 34458782
  • Jiang F  et al. Human amniotic mesenchymal stromal cells promote bone regeneration via activating endogenous regeneration. Theranostics 10:6216-6230 (2020). PubMed: 32483449
  • Wang K  et al. Statin rosuvastatin inhibits apoptosis of human coronary artery endothelial cells through upregulation of the JAK2/STAT3 signaling pathway. Mol Med Rep 22:2052-2062 (2020). PubMed: 32582964
  • Lee RJ  et al. Brain microenvironment-driven resistance to immune and targeted therapies in acral melanoma. ESMO Open 5:N/A (2020). PubMed: 32817058
  • Klein S  et al. Immune-phenotyping of pleomorphic dermal sarcomas suggests this entity as a potential candidate for immunotherapy. Cancer Immunol Immunother 68:973-982 (2019). PubMed: 30963193
View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Mandible implant)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Sodium Citrate Buffer
Permeabilization
Yes - Formalin circulation for 30 mins
Specification
Mandible implant
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Formaldehyde
Read More

DR. Pamela Yelick

Verified customer

Submitted Aug 18 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y]

 Poor
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: autoclave treatment
Permeabilization
No
Specification
spleen
Blocking step
Peroxidase-Blocking Solution, Dako REAL™ (s2023) as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 26°C
Fixative
Formalin
Read More

浩央 佐藤

Verified customer

Submitted Jul 27 2015

Immunohistochemistry (Frozen sections) abreview for Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y]

 Below Average
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.1M PBS with 3% TritonX100
Fixative
Formaldehyde
Read More

DR. Ruma Raha-Chowdhury

Verified customer

Submitted Nov 05 2014

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