Anti-MHC class I antibody [28-14-8] - Low endotoxin, Azide free (ab171283)
Key features and details
- Mouse monoclonal [28-14-8] to MHC class I - Low endotoxin, Azide free
- Suitable for: Flow Cyt, Functional Studies
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-MHC class I antibody [28-14-8] - Low endotoxin, Azide free
See all MHC class I primary antibodies -
Description
Mouse monoclonal [28-14-8] to MHC class I - Low endotoxin, Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, Functional Studiesmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Mouse splenocyte suspensions.
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General notes
Less than 0.001 ng/µg antibody, as determined by the LAL assay.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
28-14-8 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
(Final test volume = 100 uL). ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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Functional Studies |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. (Final test volume = 100 uL). ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Functional Studies
Use at an assay dependent concentration. |
Target
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Relevance
MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen. -
Cellular localization
Cell Membrane; Type I membrane protein. -
Alternative names
- A 28 antibody
- A 9 antibody
- Antigen presenting molecule antibody
see all
Datasheets and documents
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Datasheet download
References (0)
ab171283 has not yet been referenced specifically in any publications.