Overview

  • Product name
    Anti-MHC class I antibody [ER-HR 52]
    See all MHC class I primary antibodies
  • Description
    Rat monoclonal [ER-HR 52] to MHC class I
  • Host species
    Rat
  • Specificity
    Ab15681 detects MHC class I antigens of d and b haplotypes.
  • Tested applications
    Suitable for: IHC-Fr, IHC (PFA fixed)more details
    Unsuitable for: IHC-FoFr
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Murine macrophage precursor cells.

  • Positive control
    • IHC-Fr: Mouse spleen and brain tissue.
  • General notes


    Ab15681 detects MHC class I antigens and is therefore a valuable tool for studying cytotoxic T-cell interactions with class I positive antigen presenting cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 10mg/ml BSA. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Ab15681 detects MHC class I antigens and is therefore a valuable tool for studying cytotoxic T-cell interactions with class I positive antigen presenting cells.
  • Clonality
    Monoclonal
  • Clone number
    ER-HR 52
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 1 µg/ml. The determinant recognised by ab15681 is glutaraldehyde (0.05%), paraformaldehyde (1%) and acetone-resistant.
IHC (PFA fixed) 1/300. PubMed: 17446933
  • Application notes
    Is unsuitable for IHC-FoFr.
  • Target

    • Relevance
      MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
    • Cellular localization
      Cell Membrane; Type I membrane protein.
    • Alternative names
      • A 28 antibody
      • A 9 antibody
      • Antigen presenting molecule antibody
      • Aw 24 antibody
      • Aw 68 antibody
      • CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
      • H2 K1 antibody
      • H2K antibody
      • HLA A antibody
      • HLA class I histocompatibility antigen A 1 alpha chain antibody
      • HLA class I histocompatibility antigen A 11 alpha chain antibody
      • HLA class I histocompatibility antigen A 2 alpha chain antibody
      • HLA class I histocompatibility antigen A 24 alpha chain antibody
      • HLA class I histocompatibility antigen A 3 alpha chain antibody
      • HLA class I histocompatibility antigen A 30 alpha chain antibody
      • HLA class I histocompatibility antigen A 32 alpha chain antibody
      • HLA class I histocompatibility antigen A 68 alpha chain antibody
      • HLAA antibody
      • Leukocyte antigen class I A antibody
      • Major histocompatibility complex class I A antibody
      • MHC class I antigen A 1 antibody
      • MHC class I antigen A*11 antibody
      • MHC class I antigen A*2 antibody
      • MHC class I antigen A*24 antibody
      • MHC class I antigen A*3 antibody
      • MHC class I antigen A*30 antibody
      • MHC class I antigen A*32 antibody
      • MHC class I antigen A*68 antibody
      • MHC class I antigen HLA A heavy chain antibody
      • MHC class I heavy chain H2 K antibody
      see all

    Images

    • Frozen section of mouse spleen tissue stained for MHC class I using ab15681 at 1/200 dilution in immunohistochemical analysis. 

    • Immunohistochemical analysis of frozen mouse brain tissue, post-infection with Salmonella typhimurium, staining MHC Class I with ab15681.
      Tissue was blocked with 2% BSA, 10% normal rabbit serum, before incubating with primary antibody overnight. A biotinylated anti-rat IgG was used as the secondary antibody. Staining was detected using DAB.

    References

    This product has been referenced in:
    • Saranchova I  et al. Discovery of a Metastatic Immune Escape Mechanism Initiated by the Loss of Expression of the Tumour Biomarker Interleukin-33. Sci Rep 6:30555 (2016). IHC-Fr . Read more (PubMed: 27619158) »
    • Placke T  et al. Platelet-derived MHC class I confers a pseudonormal phenotype to cancer cells that subverts the antitumor reactivity of natural killer immune cells. Cancer Res 72:440-8 (2012). ICC/IF . Read more (PubMed: 22127925) »
    See all 7 Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Primary murine neurons)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    30 µg
    Treatment
    Different concentration of IFN-g for 24hrs
    Specification
    Primary murine neurons
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Mar 15 2018

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (tumor tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    Yes - 0.3% triton X-100
    Specification
    tumor tissue
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jun 14 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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