Overview

  • Product name

    Anti-MHC class I antibody [ERMP42]
    See all MHC class I primary antibodies
  • Description

    Rat monoclonal [ERMP42] to MHC class I
  • Host species

    Rat
  • Specificity

    Ab15680 detects murine MHC class I molecules on the surface of cells of the following haplotypes: H-2Fv, H-2Dd,H-2k,q,sp,r,w7,w22. MHC class I molecules of other haplotypes are not recognized by the antibody.

  • Tested applications

    Suitable for: IHC-Frmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Human
  • Immunogen

    murine macrophage precursor cells

  • Positive control

    • IHC-Fr: Mouse spleen tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: BSA, PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    ERMP42
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/400 - 1/800.

The epitope recognized by ab15680 is resistant to 0.05% glutaraldehyde, 1% paraformaldehyde and acetone.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Relevance

      MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
    • Cellular localization

      Cell Membrane; Type I membrane protein.
    • Alternative names

      • A 28 antibody
      • A 9 antibody
      • Antigen presenting molecule antibody
      • Aw 24 antibody
      • Aw 68 antibody
      • CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
      • H2 K1 antibody
      • H2K antibody
      • HLA A antibody
      • HLA class I histocompatibility antigen A 1 alpha chain antibody
      • HLA class I histocompatibility antigen A 11 alpha chain antibody
      • HLA class I histocompatibility antigen A 2 alpha chain antibody
      • HLA class I histocompatibility antigen A 24 alpha chain antibody
      • HLA class I histocompatibility antigen A 3 alpha chain antibody
      • HLA class I histocompatibility antigen A 30 alpha chain antibody
      • HLA class I histocompatibility antigen A 32 alpha chain antibody
      • HLA class I histocompatibility antigen A 68 alpha chain antibody
      • HLAA antibody
      • Leukocyte antigen class I A antibody
      • Major histocompatibility complex class I A antibody
      • MHC class I antigen A 1 antibody
      • MHC class I antigen A*11 antibody
      • MHC class I antigen A*2 antibody
      • MHC class I antigen A*24 antibody
      • MHC class I antigen A*3 antibody
      • MHC class I antigen A*30 antibody
      • MHC class I antigen A*32 antibody
      • MHC class I antigen A*68 antibody
      • MHC class I antigen HLA A heavy chain antibody
      • MHC class I heavy chain H2 K antibody
      see all

    Images

    • Frozen section of mouse spleen tissue stained for MHC class I using ab15680 at 1/400 dilution. 

    References

    This product has been referenced in:

    See all 5 Publications for this product

    Customer reviews and Q&As

    1-8 of 8 Abreviews or Q&A

    Answer



    I contacted the laboratory to clarify the specificity of Anti-MHC class I antibody [ERMP42] (ab15680) for you.

    I can confirm that the ab15680 does not react with MHC class I haplotypes in C57BL6 mice. I can recommend reviewing the datasheet of Anti-MHC class I antibody [ER-HR 52] (ab15681) on this occasion which does react with C57BL6 mice.

    Read More

    Answer

    ab15680 has indeed been tested in immunohistochemistry on paraffin slides. Although acetone fixation is recommended we have also obtained good results using paraformaldehyde as a fixative. In these cases, we suggest an antigen retrieval step using a citrate buffer pH6 for 5-20 minutes. To see the results from one of our customers using this method please visit the following link: https://www.abcam.com/MHC-class-I-antibody-ERMP42-ab15680/reviews/32211

    Read More

    Answer

    Thank you for your interesting question. We have some antibodies that recognize one species but not the other, but not all the targets for these antibodies are known to be expressed on these cell lines. What I found were two candidates:

    ab15680 - Detects MHC class I of the 3T3 haplotype (H-2q), but not human MHC.

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=15680).

    However, this antibody is not conjugated to a fluorochrome so it will require a secondary antibody such as ab98386:

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=98386).

    For detection of a protein on A549, I recommend anti-EGFR, ab130738. This is conjugated to phycoerythrin.

    Using these two antibodies, you should be able to sort A549 from 3T3. However, we do not guarantee exclusive species reactivity. In the past, it has been observed that ab15680 does not react with human cells and ab130738 does not react with mouse cells, but neither has been tested against these two particular cell lines.

    I hope this helps. Please let us know if you have any other questions.

    Read More
    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (pancreatic tumor stroma cells)
    Specification
    pancreatic tumor stroma cells
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Oct 05 2012

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (spleen)
    Specification
    spleen
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Oct 04 2012

    Question
    Answer

    Thank you for your enquiry. This antibody was tested on human tonsil and skin but unfortunately, there isn't any staining on both tissues. Therefore, we have stated on the datasheet that this product does not react with human.

    Read More

    Answer

    Thank you for your enquiry. I can confirm that indeed ab15680 will cross react with mouse and not human as it is very specific for the mouse protein. Please do not hesitate to contact us again if you require further details about ab15680,

    Read More

    Answer

    Thank you for your enquiry, we have just received the following information: We have found in our lab that the antibody does not react on routinely fixed paraffin sections (4% formalin). However, from personal communications it seems that the antibody works after more gentle fixation (1% formalin or glutaraldehyde). A higher antibody concentration than what is recommended for frozen sections would have to be used (1:20 or 1:50 and up), we suspect. We have found that this antibody stains the vast majority of cells in tissues and bone marrow smears. It also depends on the mouse strain used: ab15680 detects murine MHC class I molecules on the surface of cells of the following haplotypes: H-2Fv, H-2Dd, H-2k,q,s. A weaker reactivity is found in mouse strains with the following haplotypes: H-2 p,r,w7,w22. MHC class I molecules of other haplotypes are not recognized by the antibody. Please do not hesitate to contact us again if you need further details of wish to check whether the antibody will react in your mouse strain.

    Read More

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