Overview

  • Product name
    Anti-MHC class I antibody [ERMP42]
    See all MHC class I primary antibodies
  • Description
    Rat monoclonal [ERMP42] to MHC class I
  • Host species
    Rat
  • Specificity
    Ab15680 detects murine MHC class I molecules on the surface of cells of the following haplotypes: H-2Fv, H-2Dd,H-2k,q,sp,r,w7,w22. MHC class I molecules of other haplotypes are not recognized by the antibody.
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human
  • Immunogen

    murine macrophage precursor cells

  • Positive control
    • IHC-Fr: Mouse spleen tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: BSA, PBS
  • Concentration information loading...
  • Purity
    Affinity purified
  • Clonality
    Monoclonal
  • Clone number
    ERMP42
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/200. Acetone fixation recommended.

Target

  • Relevance
    MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
  • Cellular localization
    Cell Membrane; Type I membrane protein.
  • Alternative names
    • A 28 antibody
    • A 9 antibody
    • Antigen presenting molecule antibody
    • Aw 24 antibody
    • Aw 68 antibody
    • CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
    • H2 K1 antibody
    • H2K antibody
    • HLA A antibody
    • HLA class I histocompatibility antigen A 1 alpha chain antibody
    • HLA class I histocompatibility antigen A 11 alpha chain antibody
    • HLA class I histocompatibility antigen A 2 alpha chain antibody
    • HLA class I histocompatibility antigen A 24 alpha chain antibody
    • HLA class I histocompatibility antigen A 3 alpha chain antibody
    • HLA class I histocompatibility antigen A 30 alpha chain antibody
    • HLA class I histocompatibility antigen A 32 alpha chain antibody
    • HLA class I histocompatibility antigen A 68 alpha chain antibody
    • HLAA antibody
    • Leukocyte antigen class I A antibody
    • Major histocompatibility complex class I A antibody
    • MHC class I antigen A 1 antibody
    • MHC class I antigen A*11 antibody
    • MHC class I antigen A*2 antibody
    • MHC class I antigen A*24 antibody
    • MHC class I antigen A*3 antibody
    • MHC class I antigen A*30 antibody
    • MHC class I antigen A*32 antibody
    • MHC class I antigen A*68 antibody
    • MHC class I antigen HLA A heavy chain antibody
    • MHC class I heavy chain H2 K antibody
    see all

Images

  • Frozen section of mouse spleen tissue stained for MHC class I using ab15680 at 1/400 dilution in immunohistochemical analysis. 

References

This product has been referenced in:
  • Keilhoff G  et al. Selected gene profiles of stressed NSC-34 cells and rat spinal cord following peripheral nerve reconstruction and minocycline treatment. Exp Ther Med 11:1685-1699 (2016). IHC-FoFr ; Rat . Read more (PubMed: 27168790) »
  • Belogurov A  et al. Ubiquitin-independent proteosomal degradation of myelin basic protein contributes to development of neurodegenerative autoimmunity. FASEB J 29:1901-13 (2015). Read more (PubMed: 25634956) »
See all 2 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer



I contacted the laboratory to clarify the specificity of Anti-MHC class I antibody [ERMP42] (ab15680) for you.

I can confirm that the ab15680 does not react with MHC class I haplotypes in C57BL6 mice. I can recommend reviewing the datasheet of Anti-MHC class I antibody [ER-HR 52] (ab15681) on this occasion which does react with C57BL6 mice.

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Answer

ab15680 has indeed been tested in immunohistochemistry on paraffin slides. Although acetone fixation is recommended we have also obtained good results using paraformaldehyde as a fixative. In these cases, we suggest an antigen retrieval step using a citrate buffer pH6 for 5-20 minutes. To see the results from one of our customers using this method please visit the following link: https://www.abcam.com/MHC-class-I-antibody-ERMP42-ab15680/reviews/32211

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Answer

Thank you for your interesting question. We have some antibodies that recognize one species but not the other, but not all the targets for these antibodies are known to be expressed on these cell lines. What I found were two candidates:

ab15680 - Detects MHC class I of the 3T3 haplotype (H-2q), but not human MHC.

Click here (or use the following: https://www.abcam.com/index.html?datasheet=15680).

However, this antibody is not conjugated to a fluorochrome so it will require a secondary antibody such as ab98386:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=98386).

For detection of a protein on A549, I recommend anti-EGFR, ab130738. This is conjugated to phycoerythrin.

Using these two antibodies, you should be able to sort A549 from 3T3. However, we do not guarantee exclusive species reactivity. In the past, it has been observed that ab15680 does not react with human cells and ab130738 does not react with mouse cells, but neither has been tested against these two particular cell lines.

I hope this helps. Please let us know if you have any other questions.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreatic tumor stroma cells)
Specification
pancreatic tumor stroma cells
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (spleen)
Specification
spleen
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 04 2012

Question
Answer

Thank you for your enquiry. This antibody was tested on human tonsil and skin but unfortunately, there isn't any staining on both tissues. Therefore, we have stated on the datasheet that this product does not react with human.

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Answer

Thank you for your enquiry. I can confirm that indeed ab15680 will cross react with mouse and not human as it is very specific for the mouse protein. Please do not hesitate to contact us again if you require further details about ab15680,

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Answer

Thank you for your enquiry, we have just received the following information: We have found in our lab that the antibody does not react on routinely fixed paraffin sections (4% formalin). However, from personal communications it seems that the antibody works after more gentle fixation (1% formalin or glutaraldehyde). A higher antibody concentration than what is recommended for frozen sections would have to be used (1:20 or 1:50 and up), we suspect. We have found that this antibody stains the vast majority of cells in tissues and bone marrow smears. It also depends on the mouse strain used: ab15680 detects murine MHC class I molecules on the surface of cells of the following haplotypes: H-2Fv, H-2Dd, H-2k,q,s. A weaker reactivity is found in mouse strains with the following haplotypes: H-2 p,r,w7,w22. MHC class I molecules of other haplotypes are not recognized by the antibody. Please do not hesitate to contact us again if you need further details of wish to check whether the antibody will react in your mouse strain.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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