Overview

  • Product name
    Anti-MHC Class I H2 Kb antibody
  • Description
    Rabbit polyclonal to MHC Class I H2 Kb
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Recombinant fragment
  • Immunogen

    Synthetic peptide corresponding an internal sequence of Mouse MHC Class I H2 Kb.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purity >90%
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA 1/2000 - 1/5000.

Target

  • Relevance
    H2-Kb belongs to the family of the mouse Major Histocompatibility Complex class I receptors. These display processed antigens from the endocytosed bacteria. MHC class I proteins can form complexes with processed antigen peptides only if the antigen is synthesized in the same cell. As a consequence, T lymphocytes can only bind to class-I-positive cells that are synthesizing the antigen (e.g. virus-infected cells).
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • H2 class I histocompatibility antigen antibody
    • H2K antibody
    • H2K(B) antibody
    • H2K1 antibody
    • KB alpha chain antibody
    see all

Images

  • All lanes : Anti-MHC Class I H2 Kb antibody (ab93364) at 1 µg/ml

    Lane 1 : Whole cell lysate prepared from murine insulinoma cells MIN6 untreated.
    Lane 2 : Whole cell lysate prepared from murine insulinoma cells MIN6 treated with 60U/ml interferon-gamma for 72 hours.

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit (H+L) polyclonal at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 45 kDa (why is the actual band size different from the predicted?)


    Exposure time: 20 seconds

    See Abreview

References

This product has been referenced in:
  • Maschalidi S  et al. UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells. Nat Commun 8:1640 (2017). Read more (PubMed: 29158474) »

See 1 Publication for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Colon Cancer Cell Line- CT26)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30 µg
Specification
Mouse Colon Cancer Cell Line- CT26
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Aug 19 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.1M PBS with 3% TritonX100
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Nov 05 2014

Application
Western blot
Loading amount
1.2e+007 cells
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Mouse Tissue lysate - whole (spleenocytes)
Specification
spleenocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 17 2014

Application
Western blot
Sample
Mouse Cell lysate - whole cell (murine insulinoma cells MIN6)
Loading amount
25 µg
Specification
murine insulinoma cells MIN6
Treatment
60U/ml interferon-gamma for 72hours
Gel Running Conditions
Reduced Denaturing (10% Resolving gel, 5% stacking gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Apr 19 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate pH 4.0, 6.0 and 8.0
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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