Product nameAnti-MHC Class I H2 Kb antibody
DescriptionRabbit polyclonal to MHC Class I H2 Kb
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse, Recombinant fragment
Synthetic peptide corresponding an internal sequence of Mouse MHC Class I H2 Kb.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurity >90%
Our Abpromise guarantee covers the use of ab93364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.|
|ELISA||1/2000 - 1/5000.|
RelevanceH2-Kb belongs to the family of the mouse Major Histocompatibility Complex class I receptors. These display processed antigens from the endocytosed bacteria. MHC class I proteins can form complexes with processed antigen peptides only if the antigen is synthesized in the same cell. As a consequence, T lymphocytes can only bind to class-I-positive cells that are synthesizing the antigen (e.g. virus-infected cells).
Cellular localizationCell Membrane
- H2 class I histocompatibility antigen antibody
- H2K antibody
- H2K(B) antibody
All lanes : Anti-MHC Class I H2 Kb antibody (ab93364) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from murine insulinoma cells MIN6 untreated.
Lane 2 : Whole cell lysate prepared from murine insulinoma cells MIN6 treated with 60U/ml interferon-gamma for 72 hours.
Lysates/proteins at 25 µg per lane.
All lanes : HRP conjugated goat anti-rabbit (H+L) polyclonal at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 20 seconds