• Product name
    Anti-MHC Class I H2 Kb antibody
  • Description
    Rabbit polyclonal to MHC Class I H2 Kb
  • Host species
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Recombinant fragment
  • Immunogen

    Synthetic peptide corresponding an internal sequence of Mouse MHC Class I H2 Kb.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purity >90%
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab93364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA 1/2000 - 1/5000.


  • Relevance
    H2-Kb belongs to the family of the mouse Major Histocompatibility Complex class I receptors. These display processed antigens from the endocytosed bacteria. MHC class I proteins can form complexes with processed antigen peptides only if the antigen is synthesized in the same cell. As a consequence, T lymphocytes can only bind to class-I-positive cells that are synthesizing the antigen (e.g. virus-infected cells).
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • H2 class I histocompatibility antigen antibody
    • H2K antibody
    • H2K(B) antibody
    • H2K1 antibody
    • KB alpha chain antibody
    see all


  • All lanes : Anti-MHC Class I H2 Kb antibody (ab93364) at 1 µg/ml

    Lane 1 : Whole cell lysate prepared from murine insulinoma cells MIN6 untreated.
    Lane 2 : Whole cell lysate prepared from murine insulinoma cells MIN6 treated with 60U/ml interferon-gamma for 72 hours.

    Lysates/proteins at 25 µg per lane.

    All lanes : HRP conjugated goat anti-rabbit (H+L) polyclonal at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 45 kDa (why is the actual band size different from the predicted?)

    Exposure time: 20 seconds

    See Abreview


This product has been referenced in:
  • Maschalidi S  et al. UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells. Nat Commun 8:1640 (2017). Read more (PubMed: 29158474) »

See 1 Publication for this product

Customer reviews and Q&As

we have issued a free of charge replacement.

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Your credit note ID is 23807.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used i...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

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DISCOUNT CODE: xx Ablaufdatum: 20. Juli 2012

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab93364 im Western blot gegen das endogene Mausprotein zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Guts...

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Thank you for contacting us. We have only tested this antibody with recombinant fragment which we used as immunogen. We have not tested it using endogenous protein or lysates. This is the reason why the mouse species is predicted not tested. Ab...

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Thank you for contacting us. I can confirm that using this MHC Class I H2 Kb antibody ab93364 a lower band can be expected at around 40 kDa which is due to the non-glycosylated form of the protein. The upper band is the glycosylated mature prot...

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