Product nameAnti-MHC Class II antibody
See all MHC Class II primary antibodies
DescriptionRabbit polyclonal to MHC Class II
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human MHC Class II aa 30-225. Extracellular domain.
RATPENYLFQGRQECYAFNGTQRFLERYIYNREEFARFDSDVGEFRAVTE LGRPAAEYWN SQKDILEEKRAVPDRMCRHNYELGGPMTLQRRVQPRVN VSPSKKGPLQHHNLLVCHVTDF YPGSIQVRWFLNGQEETAGVVSTNLI RNGDWTFQILVMLEMTPQQGDVYTCQVEHTSLDS PVTVEWKAQSDSAR SK
Database link: P04440
- WB: U-937 and THP-1 cell lysates; mouse spleen and thymus tissue lysates; IHC: Human lymph node and stomach tissues.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab180779 in the following tested applications.
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/2000. Predicted molecular weight: 29 kDa.|
FunctionBinds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
Sequence similaritiesBelongs to the MHC class II family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
- Information by UniProt
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Paraffin-embedded human stomach tissue stained for MHC Class II using ab180779 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-MHC Class II antibody (ab180779) at 1/1000 dilution
Lane 1 : U-937 cell lysate
Lane 2 : THP-1 cell lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Mouse thymus tissue lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Predicted band size: 29 kDa
Blocking buffer: 3% nonfat dry milk in TBST.
Paraffin-embedded human lymph node tissue stained for MHC Class II using ab180779 at 1/100 dilution in immunohistochemical analysis.
ab180779 has been referenced in 7 publications.
- Bhat SA et al. AT2R Activation Prevents Microglia Pro-inflammatory Activation in a NOX-Dependent Manner: Inhibition of PKC Activation and p47phox Phosphorylation by PP2A. Mol Neurobiol 56:3005-3023 (2019). PubMed: 30076526
- Larssen P et al. Allogenicity Boosts Extracellular Vesicle-Induced Antigen-Specific Immunity and Mediates Tumor Protection and Long-Term Memory In Vivo. J Immunol 203:825-834 (2019). PubMed: 31292216
- Biglari S et al. Simulating Inflammation in a Wound Microenvironment Using a Dermal Wound-on-a-Chip Model. Adv Healthc Mater 8:e1801307 (2019). PubMed: 30511808
- Zong J et al. Nuclear Localization Leucine-Rich-Repeat Protein 1 Deficiency Protects Against Cardiac Hypertrophy by Pressure Overload. Cell Physiol Biochem 48:75-86 (2018). PubMed: 30001530
- Ge J et al. Gold and gold-silver alloy nanoparticles enhance the myogenic differentiation of myoblasts through p38 MAPK signaling pathway and promote in vivo skeletal muscle regeneration. Biomaterials 175:19-29 (2018). WB . PubMed: 29793089
- Yuan T et al. Low-density lipoprotein receptor-related protein 6 regulates alternative pre-mRNA splicing. J Cell Mol Med 22:4653-4663 (2018). PubMed: 30070011
- Nowyhed HN et al. ATP Binding Cassette Transporter ABCA7 Regulates NKT Cell Development and Function by Controlling CD1d Expression and Lipid Raft Content. Sci Rep 7:40273 (2017). PubMed: 28091533