Anti-MHC Class II antibody [M5/114] (ab139365)
Key features and details
- Rat monoclonal [M5/114] to MHC Class II
- Suitable for: IP, WB, Flow Cyt, Blocking, Functional Studies
- Reacts with: Mouse
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-MHC Class II antibody [M5/114]
See all MHC Class II primary antibodies -
Description
Rat monoclonal [M5/114] to MHC Class II -
Host species
Rat -
Specificity
ab139365 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. ab139365 can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes. -
Tested applications
Suitable for: IP, WB, Flow Cyt, Blocking, Functional Studiesmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse MHC Class II. Activated C57BL/6 Mouse spleen cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.40
Constituent: 99% PBS
PBS is Azide free -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity is > 95% by SDS-PAGE and 0.2 µm filter sterilized. -
Clonality
Monoclonal -
Clone number
M5/114 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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Blocking |
Use at an assay dependent concentration.
Blocking of T cell proliferative responses. |
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Functional Studies |
Use at an assay dependent concentration.
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Notes |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Blocking
Use at an assay dependent concentration. Blocking of T cell proliferative responses. |
Functional Studies
Use at an assay dependent concentration. |
Target
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Function
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. -
Sequence similarities
Belongs to the MHC class II family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation. - Information by UniProt
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Database links
- Entrez Gene: 100504404 Mouse
- SwissProt: P01904 Mouse
- SwissProt: P04224 Mouse
- SwissProt: P14439 Mouse
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Alternative names
- D6S221E antibody
- DMA antibody
- DMB antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab139365 has been referenced in 8 publications.
- Hwang M et al. Trem2 deficiency impairs recovery and phagocytosis and dysregulates myeloid gene expression during virus-induced demyelination. J Neuroinflammation 19:267 (2022). PubMed: 36333761
- Whary MT et al. Effects of Colonization of Gnotobiotic Swiss Webster Mice with Helicobacter bilis. Comp Med 70:216-232 (2020). PubMed: 32349859
- Yan A et al. Partial Depletion of Peripheral M1 Macrophages Reverses Motor Deficits in MPTP-Treated Mouse by Suppressing Neuroinflammation and Dopaminergic Neurodegeneration. Front Aging Neurosci 10:160 (2018). PubMed: 29922149
- Zhou X et al. Analysis of the direct injury effector of oligodendroglia cells or myelin sheath in an experimental allergic encephalomyelitis model induced by the MOG35-55 peptide. Mol Med Rep 12:7425-32 (2015). PubMed: 26459920
- Puntambekar SS et al. Interleukin-10 is a critical regulator of white matter lesion containment following viral induced demyelination. Glia 63:2106-2120 (2015). PubMed: 26132901
- Mori H et al. Introduction of Zinc-salt Fixation for Effective Detection of Immune Cell-related Markers by Immunohistochemistry. Toxicol Pathol 43:883-9 (2015). PubMed: 26157038
- Otsuka A et al. Possible new therapeutic strategy to regulate atopic dermatitis through upregulating filaggrin expression. J Allergy Clin Immunol 133:139-46.e1-10 (2014). PubMed: 24055295
- Jin Y et al. Regulation of SIV antigen-specific CD4+ T cellular immunity via autophagosome-mediated MHC II molecule-targeting antigen presentation in mice. PLoS One 9:e93143 (2014). IF ; Human . PubMed: 24671203