Recombinant
RabMAb

Recombinant Anti-MHC Class II beta antibody [EPR11227] (ab170867)

Overview

  • Product name

    Anti-MHC Class II beta antibody [EPR11227]
    See all MHC Class II beta primary antibodies
  • Description

    Rabbit monoclonal [EPR11227] to MHC Class II beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MHC Class II beta (Cysteine residue). The exact sequence is proprietary.
    Database link: P04440

  • Positive control

    • Human kidney and uterus tissues; Human tonsil, thymus and Raji lysates; Raji cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11227
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Relevance

      Major Histocompatibility Class II molecules are primarily expressed on B-cells and antigen presenting cells (APCs). They are composed of two polypeptide chains. These polypeptides (alpha and beta) are about 230 and 240 amino acids long, respectively, and are glycosylated. These polypeptides fold into two separate domains; the non-polymorphic alpha chain and the polymorphic beta chain. The antigen binding region of the molecule is bounded by a beta-pleated sheet on the bottom and two alpha helices on the sides, and is capable of binding (via non-covalent interactions) a small peptide of about 10 amino acids.
    • Cellular localization

      Cell Membrane
    • Database links

    • Alternative names

      • DPB1 antibody
      • HLA-DP antibody
      • HLA-DP1B antibody
      • HLA-DPB antibody
      • Major Histocompatibility Complex Class II beta antibody
      see all

    Images

    • Immunofluorescent analysis of Raji cells labeling MHC Class II beta with ab170867 at 1/100 dilution (green). DAPI nuclear staining (blue).

    • Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human uterus tissue labeling MHC Class II beta with ab170867 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of Formalin-fixed, paraffin-embedded human lymphoid follicle labeling MHC Class II beta with ab170867 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • All lanes : Anti-MHC Class II beta antibody [EPR11227] (ab170867) at 1/1000 dilution

      Lane 1 : Human tonsil lysates
      Lane 2 : Human thymus lysates
      Lane 3 : Raji lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 29 kDa

    References

    This product has been referenced in:

    • Yuan Y  et al. Long non-coding RNA cytoskeleton regulator RNA (CYTOR) modulates pathological cardiac hypertrophy through miR-155-mediated IKKi signaling. Biochim Biophys Acta Mol Basis Dis 1865:1421-1427 (2019). Read more (PubMed: 30794866) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170867.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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