Key features and details
- Mouse monoclonal [14-4-4S] to MHC class II I E kappa
- Suitable for: Functional Studies, Flow Cyt, IP, IHC-Fr, Blocking
- Reacts with: Mouse
- Isotype: IgG2a
Product nameAnti-MHC class II I E kappa antibody [14-4-4S]
See all MHC class II I E kappa primary antibodies
DescriptionMouse monoclonal [14-4-4S] to MHC class II I E kappa
SpecificityThis antibody is specific to an epitope on mouse I-E? MHC class II alloantigen. The antibody reacts with the I-E? class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. The antibody has also been reported to cross react with the rat class II alloantigen RT1D.
Tested applicationsSuitable for: Functional Studies, Flow Cyt, IP, IHC-Fr, Blockingmore details
Species reactivityReacts with: Mouse
Tissue/ cell preparation : C3H mouse skin graft and splenocytes.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8.20
Constituent: 100% Borate buffered saline
No preservatives or amine-containing buffer salts added.
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab25681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.
In vitro induction of antigen specific B cell differentiation. Complement dependent cytotoxicity.
|Flow Cyt||Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.
|Blocking||Use at an assay dependent concentration.
In vitro blocking of antigen presentation.
RelevanceA major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
Cellular localizationType I membrane protein
- H2 Ea antibody
- H2-Ea antibody
ab25681 has been referenced in 5 publications.
- Farr A et al. Differential expression of CLIP:MHC class II and conventional endogenous peptide:MHC class II complexes by thymic epithelial cells and peripheral antigen-presenting cells. Eur J Immunol 26:3185-93 (1996). PubMed: 8977321
- Roy M et al. Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses. Eur J Immunol 25:596-603 (1995). PubMed: 7533092
- Neiss U & Reske K Non-coordinate synthesis of MHC class II proteins and invariant chains by epidermal Langerhans cells derived from short-term in vitro culture. Int Immunol 6:61-71 (1994). PubMed: 8148327
- Harton JA et al. Structure function analysis of the H-2 Abp gene. Immunogenetics 34:358-65 (1991). PubMed: 1748486
- Ozato K et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol 124:533-40 (1980). PubMed: 7188699