Overview

  • Product name

    Anti-MHC class II I E kappa antibody [14-4-4S] (Biotin)
    See all MHC class II I E kappa primary antibodies
  • Description

    Mouse monoclonal [14-4-4S] to MHC class II I E kappa (Biotin)
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Specificity

    ab93504 reacts with the mouse and rat MHC Class II, I-E subregion-encoded glycoprotein in mice strains of H-2k, H-2d, H-2p and H-2r. H-2f and H-2s haplotypes do not express the I-E antigen.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Tissue/ cell preparation: C3H mouse skin graft and splenocytes

  • Positive control

    • Mouse splenocytes
  • General notes


    The 14-4-4S monoclonal antibody has been reported to block antigen presentation in in vitro assays.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.87% Sodium chloride, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Primary antibody notes

    The 14-4-4S monoclonal antibody has been reported to block antigen presentation in in vitro assays.
  • Clonality

    Monoclonal
  • Clone number

    14-4-4S
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab18454 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
  • Cellular localization

    Type I membrane protein
  • Alternative names

    • H2 Ea antibody
    • H2-Ea antibody

References

This product has been referenced in:

  • Spencer JS  et al. Expression and function of mixed isotype MHC class II molecules in normal mice. J Immunol 151:6822-32 (1993). Read more (PubMed: 8258692) »
  • Pruitt SK  et al. The effect of xenoreactive antibody and B cell depletion on hyperacute rejection of guinea pig-to-rat cardiac xenografts. Transplantation 56:1318-24 (1993). Read more (PubMed: 8278996) »
See all 4 Publications for this product

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