Key features and details
- Mouse monoclonal [14-4-4S] to MHC class II I E kappa (FITC)
- Suitable for: Flow Cyt, IP, Blocking, IHC-Fr, Functional Studies
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Product nameAnti-MHC class II I E kappa antibody [14-4-4S] (FITC)
See all MHC class II I E kappa primary antibodies
DescriptionMouse monoclonal [14-4-4S] to MHC class II I E kappa (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Specificityab25023 recognises MHC class II I E kappa alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. It cross reacts with alloantigen RT1D. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express this antigen.
Tested applicationsSuitable for: Flow Cyt, IP, Blocking, IHC-Fr, Functional Studiesmore details
Species reactivityReacts with: Mouse
Tissue/ cell preparation: C3H mouse skin graft and splenocytes.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
Light chain typekappa
Our Abpromise guarantee covers the use of ab25023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 1µg for 106 cells.
FuncS: Use at an assay dependent dilution for complement dependent cytotoxicity studies and in vitro induction of antigen specific B cell differentiation.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceA major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
Cellular localizationType I membrane protein
- H2 Ea antibody
- H2-Ea antibody
ab25023 has been referenced in 1 publication.
- Guan J et al. Enhanced immunocompatibility of ligand-targeted liposomes by attenuating natural IgM absorption. Nat Commun 9:2982 (2018). PubMed: 30061672