Key features and details
- Mouse monoclonal [14-4-4S] to MHC class II I E kappa (PE)
- Suitable for: Flow Cyt, IP, IHC-Fr, Blocking
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Product nameAnti-MHC class II I E kappa antibody [14-4-4S] (PE)
See all MHC class II I E kappa primary antibodies
DescriptionMouse monoclonal [14-4-4S] to MHC class II I E kappa (PE)
ConjugationPE. Ex: 488nm, Em: 575nm
SpecificityThis antibody is specific to an epitope on mouse I-E? MHC class II alloantigen. The antibody reacts with the I-E? class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. The antibody has also been reported to crossreact with the rat class II alloantigen RT1D.
Tested applicationsSuitable for: Flow Cyt, IP, IHC-Fr, Blockingmore details
Species reactivityReacts with: Mouse
Tissue/ cell preparation: C3H mouse skin graft and splenocytes.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Also contains a stabilizing agent.
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab25585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr(cetone fixed): Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
BL: Use at an assay dependent dilution for in vitro blocking of antigen presentation.
Complement dependent cytotoxicity: Use at an assay dependent dilution.
In vitro induction of antigen specific B cell differentiation: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceA major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
Cellular localizationType I membrane protein
- H2 Ea antibody
- H2-Ea antibody
ab25585 has been referenced in 5 publications.
- Farr A et al. Differential expression of CLIP:MHC class II and conventional endogenous peptide:MHC class II complexes by thymic epithelial cells and peripheral antigen-presenting cells. Eur J Immunol 26:3185-93 (1996). PubMed: 8977321
- Roy M et al. Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses. Eur J Immunol 25:596-603 (1995). PubMed: 7533092
- Neiss U & Reske K Non-coordinate synthesis of MHC class II proteins and invariant chains by epidermal Langerhans cells derived from short-term in vitro culture. Int Immunol 6:61-71 (1994). PubMed: 8148327
- Harton JA et al. Structure function analysis of the H-2 Abp gene. Immunogenetics 34:358-65 (1991). PubMed: 1748486
- Ozato K et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol 124:533-40 (1980). PubMed: 7188699