Overview

  • Product name
    Anti-MHF1 antibody
  • Description
    Mouse polyclonal to MHF1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-138 of Human MHF1 (NP_954988.1).

  • Positive control
    • MHF1 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab169385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa.

Target

  • Function
    DNA-binding component of the FA core complex involved in DNA damage repair and genome maintenance. Required for optimal chromatin association of the FA core complex. Required for efficient damage-induced monoubiquitination and focus formation of FANCD2. Stabilizes FAAD24, FANCM and STRA13/CENPX in the FA core complex. Plays a role in DNA interstrand cross-linking (ICL) repair and in recorery of replication forks stalled by topoisomerase I-DNA cleavage intermediates induced by camptothecin. As a component of the APITD1/CENPS complex, is also essential for the stable assembly of the outer kinetchore. Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the TAF9 family.
  • Cellular localization
    Nucleus. Chromosome > centromere. Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • APITD1 antibody
    • Apoptosis-inducing TAF9-like domain-containing protein 1 antibody
    • apoptosis-inducing, TAF9-like domain 1 antibody
    • CENP-S antibody
    • CENPS antibody
    • CENPS_HUMAN antibody
    • Centromere protein S antibody
    • FAAP16 antibody
    • FANCM-interacting histone fold protein 1 antibody
    • Fanconi anemia-associated polypeptide of 16 kDa antibody
    • MGC32686 antibody
    • MHF 1 antibody
    see all

Images

  • All lanes : Anti-MHF1 antibody (ab169385) at 1 µg/ml

    Lane 1 : MHF1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 16 kDa

References

This product has been referenced in:
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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