Product nameAnti-Mib1/Mindbomb antibody
See all Mib1/Mindbomb primary antibodies
DescriptionGoat polyclonal to Mib1/Mindbomb
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Xenopus laevis, Zebrafish
- WB: Human Mib1/Mindbomb with Myc and DYKDDDDK-tag transfected HEK-293 cell lysate. IHC-P: Human cerebral cortex tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab240485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 110 kDa.|
FunctionE3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
Tissue specificityWidely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 9 ANK repeats.
Contains 2 MIB/HERC2 domains.
Contains 3 RING-type zinc fingers.
Contains 1 ZZ-type zinc finger.
modificationsUbiquitinated. Possibly via autoubiquitination.
Cellular localizationCytoplasm. Cell membrane. Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear.
- Information by UniProt
- DAPK-interacting protein 1 antibody
- Dip 1 antibody
- DIP-1 antibody
Lanes 1-2 : Anti-Mib1/Mindbomb antibody (ab240485) at 1 µg/ml
Lane 3 : Anti-Myc tag at 1/1000 dilution
Lane 1 : Human Mib1/Mindbomb with Myc and DYKDDDDK-tag transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate in RIPA buffer
Lane 2 : Mock transfected HEK-293 cell lysate in RIPA buffer
Lane 3 : Human Mib1/Mindbomb with Myc and DYKDDDDK-tag transfected HEK-293 cell lysate in RIPA buffer
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 110 kDa
Primary incubation for 1 hour.
In transfected HEK-293 transiently expressing human Mib1/Mindbomb (myc and DYKDDDDK tagged), a band of approx. 135kDa is observed.
Paraffin-embedded human cerebral cortex tissue stained for Mib1/Mindbomb using ab240485 at 2 μg/ml in immunohistochemical analysis. HRP staining.
ab240485 has not yet been referenced specifically in any publications.