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    mib1mindbomb-antibody-ab74134.pdf

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Stem Cells Signaling Pathways Notch Surface Molecules
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Anti-Mib1/Mindbomb antibody (ab74134)

  • Datasheet
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Western blot - Anti-Mib1/Mindbomb antibody (ab74134)

    Key features and details

    • Rabbit polyclonal to Mib1/Mindbomb
    • Suitable for: WB
    • Reacts with: Drosophila melanogaster
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Mib1/Mindbomb antibody
      See all Mib1/Mindbomb primary antibodies
    • Description

      Rabbit polyclonal to Mib1/Mindbomb
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Drosophila melanogaster
    • Immunogen

      Synthetic peptide derived from the N terminal domain of Drosophila melanogaster (Fruit fly) Mib1/Mindbomb.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Constituent: Whole serum
    • Concentration information loading...
    • Purity

      Whole antiserum
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Stem Cells
      • Signaling Pathways
      • Notch
      • Surface Molecules
      • Stem Cells
      • Signaling Pathways
      • Notch
      • Cytoplasmic

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    Our Abpromise guarantee covers the use of ab74134 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/2000. Predicted molecular weight: 130 kDa.

    Target

    • Function

      E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
    • Tissue specificity

      Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Contains 9 ANK repeats.
      Contains 2 MIB/HERC2 domains.
      Contains 3 RING-type zinc fingers.
      Contains 1 ZZ-type zinc finger.
    • Post-translational
      modifications

      Ubiquitinated. Possibly via autoubiquitination.
    • Cellular localization

      Cytoplasm. Cell membrane. Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear.
    • Target information above from: UniProt accession Q86YT6 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • DAPK-interacting protein 1 antibody
      • Dip 1 antibody
      • DIP-1 antibody
      • Dip1 antibody
      • E3 ubiquitin protein ligase MIB 1 antibody
      • E3 ubiquitin protein ligase MIB1 antibody
      • E3 ubiquitin-protein ligase mib1 antibody
      • KIAA1323 antibody
      • LVNC7 antibody
      • MIB antibody
      • mib1 antibody
      • MIB1_HUMAN antibody
      • Mind bomb homolog 1 antibody
      • Mindbomb E3 ubiquitin protein ligase 1 antibody
      • Ubiquitin ligase mind bomb antibody
      • Zinc finger ZZ type with ankyrin repeat domain protein 2 antibody
      • ZZANK2 antibody
      • ZZZ6 antibody
      see all

    Images

    • Western blot - Anti-Mib1/Mindbomb antibody (ab74134)
      Western blot - Anti-Mib1/Mindbomb antibody (ab74134)
      Anti-Mib1/Mindbomb antibody (ab74134) + S2 cells

      Predicted band size: 130 kDa
      Observed band size: 130 kDa

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab74134? Please let us know so that we can cite the reference in this datasheet.

    ab74134 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunohistochemistry (Frozen sections) abreview for Anti-Mib1/Mindbomb antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 25% · Temperature: 23°C
    Sample
    Fruit fly (Drosophila melanogaster) Tissue (Pupae, notum, epithelial)
    Specification
    Pupae, notum, epithelial
    Permeabilization
    Yes - PBST
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 04 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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