Anti-Mib1/Mindbomb antibody (ab74134)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Mib1/Mindbomb antibody
See all Mib1/Mindbomb primary antibodies -
Description
Rabbit polyclonal to Mib1/Mindbomb -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IHC-Frmore details -
Species reactivity
Reacts with: Drosophila melanogaster -
Immunogen
Synthetic peptide derived from the N terminal domain of Drosophila melanogaster (Fruit fly) Mib1/Mindbomb.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab74134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 130 kDa. | |
| ICC | Use at an assay dependent concentration. | |
| IHC-Fr | 1/50 - 1/100. |
Target
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Function
E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. -
Tissue specificity
Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 9 ANK repeats.
Contains 2 MIB/HERC2 domains.
Contains 3 RING-type zinc fingers.
Contains 1 ZZ-type zinc finger. -
Post-translational
modificationsUbiquitinated. Possibly via autoubiquitination. -
Cellular localization
Cytoplasm. Cell membrane. Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear. - Information by UniProt
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Alternative names
- DAPK-interacting protein 1 antibody
- Dip 1 antibody
- DIP-1 antibody
see all
Images
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Western blot - Anti-Mib1/Mindbomb antibody (ab74134)Anti-Mib1/Mindbomb antibody (ab74134) + S2 cells
Predicted band size: 130 kDa
Observed band size: 130 kDa
Protocols
Datasheets and documents
References
ab74134 has not yet been referenced specifically in any publications.
