Anti-Mib1/Mindbomb antibody (ab74134)
Key features and details
- Rabbit polyclonal to Mib1/Mindbomb
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Overview
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Product name
Anti-Mib1/Mindbomb antibody
See all Mib1/Mindbomb primary antibodies -
Description
Rabbit polyclonal to Mib1/Mindbomb -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Drosophila melanogaster -
Immunogen
Synthetic peptide derived from the N terminal domain of Drosophila melanogaster (Fruit fly) Mib1/Mindbomb.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab74134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/2000. Predicted molecular weight: 130 kDa. |
Target
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Function
E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. -
Tissue specificity
Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 9 ANK repeats.
Contains 2 MIB/HERC2 domains.
Contains 3 RING-type zinc fingers.
Contains 1 ZZ-type zinc finger. -
Post-translational
modificationsUbiquitinated. Possibly via autoubiquitination. -
Cellular localization
Cytoplasm. Cell membrane. Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear. - Information by UniProt
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Alternative names
- DAPK-interacting protein 1 antibody
- Dip 1 antibody
- DIP-1 antibody
see all
Images
Protocols
Datasheets and documents
References (0)
ab74134 has not yet been referenced specifically in any publications.