Key features and details
- Rabbit polyclonal to Mib1/Mindbomb - C-terminal
- Suitable for: WB, ICC/IF, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Mib1/Mindbomb antibody - C-terminal
See all Mib1/Mindbomb primary antibodies
DescriptionRabbit polyclonal to Mib1/Mindbomb - C-terminal
Tested applicationsSuitable for: WB, ICC/IF, ICCmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Mib1/Mindbomb (C terminal). The exact sequence is proprietary. 19 amino acid peptide.
Database link: Q86YT6
- A431 cells and cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191839 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 110 kDa).|
|ICC/IF||Use a concentration of 20 µg/ml.|
|ICC||Use a concentration of 5 µg/ml.|
FunctionE3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
Tissue specificityWidely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 9 ANK repeats.
Contains 2 MIB/HERC2 domains.
Contains 3 RING-type zinc fingers.
Contains 1 ZZ-type zinc finger.
modificationsUbiquitinated. Possibly via autoubiquitination.
Cellular localizationCytoplasm. Cell membrane. Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear.
- Information by UniProt
- DAPK-interacting protein 1 antibody
- Dip 1 antibody
- DIP-1 antibody
ab191839 has not yet been referenced specifically in any publications.