Overview

  • Product name

    Anti-Mib1/Mindbomb antibody - C-terminal
    See all Mib1/Mindbomb primary antibodies
  • Description

    Rabbit polyclonal to Mib1/Mindbomb - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Mib1/Mindbomb (C terminal). The exact sequence is proprietary. 19 amino acid peptide.
    Database link: Q86YT6

  • Positive control

    • A431 cells and cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab191839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 110 kDa).
ICC/IF Use a concentration of 20 µg/ml.
ICC Use a concentration of 5 µg/ml.

Target

  • Function

    E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
  • Tissue specificity

    Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 9 ANK repeats.
    Contains 2 MIB/HERC2 domains.
    Contains 3 RING-type zinc fingers.
    Contains 1 ZZ-type zinc finger.
  • Post-translational
    modifications

    Ubiquitinated. Possibly via autoubiquitination.
  • Cellular localization

    Cytoplasm. Cell membrane. Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • DAPK-interacting protein 1 antibody
    • Dip 1 antibody
    • DIP-1 antibody
    • Dip1 antibody
    • E3 ubiquitin protein ligase MIB 1 antibody
    • E3 ubiquitin protein ligase MIB1 antibody
    • E3 ubiquitin-protein ligase mib1 antibody
    • KIAA1323 antibody
    • LVNC7 antibody
    • MIB antibody
    • mib1 antibody
    • MIB1_HUMAN antibody
    • Mind bomb homolog 1 antibody
    • Mindbomb E3 ubiquitin protein ligase 1 antibody
    • Ubiquitin ligase mind bomb antibody
    • Zinc finger ZZ type with ankyrin repeat domain protein 2 antibody
    • ZZANK2 antibody
    • ZZZ6 antibody
    see all

Images

  • Anti-Mib1/Mindbomb antibody - C-terminal (ab191839) at 1 µg/ml + A431 cell lysate at 15 µg

    Predicted band size: 110 kDa

  • Immunofluorescent analysis of A431 cells labeling Mib1/Mindbomb with ab191839 at 20 µg/ml

  • Immunocytochemical analysis of A431 cells labeling Mib1/Mindbomb with ab191839 at 5 µg/ml.

References

ab191839 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191839.
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