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    mic19-antibody-ab154500.pdf

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Tags & Cell Markers Subcellular Markers Organelles Mitochondria
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Anti-MIC19 antibody (ab154500)

  • Datasheet
  • SDS
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Western blot - Anti-MIC19 antibody (ab154500)
  • Western blot - Anti-MIC19 antibody (ab154500)
  • Western blot - Anti-MIC19 antibody (ab154500)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab154500)

Key features and details

  • Rabbit polyclonal to MIC19
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human MIC19 protein (ab171478)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-MIC19 antibody
    See all MIC19 primary antibodies
  • Description

    Rabbit polyclonal to MIC19
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to Human MIC19 aa 1-214.
    Database link: Q9NX63

  • Positive control

    • 293T, A431, Jurkat, Raji and mouse liver lysates; Hela xenograft
  • General notes

     This product was previously labelled as CHCHD3

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse liver tissue lysate - total protein (ab29301)
    • Jurkat whole cell lysate (ab30128)
    • A431 whole cell lysate (ab30132)
    • Jurkat whole cell lysate (ab7899)
    • A431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human MIC19 protein (ab171478)

Applications

Our Abpromise guarantee covers the use of ab154500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Required for maintenance of mitochondrial crista integrity and mitochondrial function. May act as a scaffolding protein that stabilizes protein complexes involved in crista architecture and protein import. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription.
  • Tissue specificity

    Detected at low levels in brain, placenta, lung, liver, kidney and pancreas with increased levels in heart and skeletal muscle. Higher expression in primary lung cancers than in normal lung tissue.
  • Sequence similarities

    Contains 1 CHCH domain.
  • Cellular localization

    Mitochondrion inner membrane. Cytoplasm. Nucleus.
  • Target information above from: UniProt accession Q9NX63 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 616477 Cow
    • Entrez Gene: 54927 Human
    • Entrez Gene: 66075 Mouse
    • Omim: 613748 Human
    • SwissProt: Q5E9D3 Cow
    • SwissProt: Q9NX63 Human
    • SwissProt: Q9CRB9 Mouse
    • Unigene: 655010 Human
    • Unigene: 21501 Mouse
    see all
  • Alternative names

    • CHCH3_HUMAN antibody
    • CHCHD 3 antibody
    • Chchd3 antibody
    • Coiled coil helix coiled coil helix domain containing 3 antibody
    • Coiled coil helix coiled coil helix domain containing protein 3 mitochondrial antibody
    • Coiled-coil-helix-coiled-coil-helix domain-containing protein 3 antibody
    • FLJ20420 antibody
    • MINOS3 antibody
    • mitochondrial antibody
    • Mitochondrial inner membrane organizing system 3 antibody
    • PPP1R22 antibody
    • Protein phosphatase 1 regulatory subunit 22 antibody
    see all

Images

  • Western blot - Anti-MIC19 antibody (ab154500)
    Western blot - Anti-MIC19 antibody (ab154500)
    All lanes : Anti-MIC19 antibody (ab154500) at 1/2000 dilution

    Lane 1 : WT 293T cell extracts
    Lane 2 : MIC19 Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 26 kDa

  • Western blot - Anti-MIC19 antibody (ab154500)
    Western blot - Anti-MIC19 antibody (ab154500)
    All lanes : Anti-MIC19 antibody (ab154500) at 1/5000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Western blot - Anti-MIC19 antibody (ab154500)
    Western blot - Anti-MIC19 antibody (ab154500)
    Anti-MIC19 antibody (ab154500) at 1/1000 dilution + mouse liver whole cell lysate at 50 µg

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab154500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab154500)

    Immunohistochemical analysis of paraffin-embedded Hela xenograft, labeling MIC19 with ab154500 at 1/500 dilution.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab154500? Please let us know so that we can cite the reference in this datasheet.

    ab154500 has been referenced in 1 publication.

    • Habich M  et al. Vectorial Import via a Metastable Disulfide-Linked Complex Allows for a Quality Control Step and Import by the Mitochondrial Disulfide Relay. Cell Rep 26:759-774.e5 (2019). PubMed: 30650365

    Customer reviews and Q&As

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    Please use the links above to contact us or submit feedback about this product.

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