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Anti-MIC19 antibody (ab224565)

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Abpromise

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Find out more.

Western blot - Anti-MIC19 antibody (ab224565)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)
  • Immunocytochemistry/ Immunofluorescence - Anti-MIC19 antibody (ab224565)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)
  • Western blot - Anti-MIC19 antibody (ab224565)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)
  • Western blot - Anti-MIC19 antibody (ab224565)

Key features and details

  • Rabbit polyclonal to MIC19
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-MIC19 antibody
    See all MIC19 primary antibodies
  • Description

    Rabbit polyclonal to MIC19
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to Human MIC19 aa 15-89.
    Sequence:

    DENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSGAYGASVSDEELKR RVAEELALEQAKKESEDQKRLKQAK


    Database link: Q9NX63
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human stomach, kidney, duodenum, skeletal muscle and liver tissue. ICC/IF: U-2 OS cells. WB: HEK-293, NIH/3T3 and NBT-II cell lysates.
  • General notes

     This product was previously labelled as CHCHD3

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • NIH 3T3 whole cell lysate (ab7179)
    • HEK293 whole cell lysate (ab7902)
  • Recombinant Protein

    • Recombinant Human MIC19 protein (ab171478)
  • Related Products

    • Recombinant Human MIC19 protein (ab171478)

Applications

Our Abpromise guarantee covers the use of ab224565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

WB Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 26 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Required for maintenance of mitochondrial crista integrity and mitochondrial function. May act as a scaffolding protein that stabilizes protein complexes involved in crista architecture and protein import. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription.
  • Tissue specificity

    Detected at low levels in brain, placenta, lung, liver, kidney and pancreas with increased levels in heart and skeletal muscle. Higher expression in primary lung cancers than in normal lung tissue.
  • Sequence similarities

    Contains 1 CHCH domain.
  • Cellular localization

    Mitochondrion inner membrane. Cytoplasm. Nucleus.
  • Target information above from: UniProt accession Q9NX63 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 616477 Cow
    • Entrez Gene: 54927 Human
    • Entrez Gene: 66075 Mouse
    • Entrez Gene: 296966 Rat
    • Omim: 613748 Human
    • SwissProt: Q5E9D3 Cow
    • SwissProt: Q9NX63 Human
    • SwissProt: Q9CRB9 Mouse
    • Unigene: 655010 Human
    • Unigene: 21501 Mouse
    • Unigene: 106389 Rat
    see all
  • Alternative names

    • CHCH3_HUMAN antibody
    • CHCHD 3 antibody
    • Chchd3 antibody
    • Coiled coil helix coiled coil helix domain containing 3 antibody
    • Coiled coil helix coiled coil helix domain containing protein 3 mitochondrial antibody
    • Coiled-coil-helix-coiled-coil-helix domain-containing protein 3 antibody
    • FLJ20420 antibody
    • MINOS3 antibody
    • mitochondrial antibody
    • Mitochondrial inner membrane organizing system 3 antibody
    • PPP1R22 antibody
    • Protein phosphatase 1 regulatory subunit 22 antibody
    see all

Images

  • Western blot - Anti-MIC19 antibody (ab224565)
    Western blot - Anti-MIC19 antibody (ab224565)
    Anti-MIC19 antibody (ab224565) at 1/100 dilution + HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

    Developed using the ECL technique.

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)

    Immunohistochemical analysis of human duodenum tissue labelling MIC19 with ab224565 at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MIC19 antibody (ab224565)
    Immunocytochemistry/ Immunofluorescence - Anti-MIC19 antibody (ab224565)

    PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for MIC19 (green) using ab224565 at 4 µg/ml in ICC/IF.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)

    Immunohistochemical analysis of human kidney tissue labelling MIC19 with ab224565 at 1/500 dilution.

  • Western blot - Anti-MIC19 antibody (ab224565)
    Western blot - Anti-MIC19 antibody (ab224565)
    All lanes : Anti-MIC19 antibody (ab224565) at 0.4 µg/ml

    Lane 1 : U-87MG ATCC cells transfected with control siRNA
    Lanes 2-3 : U-87MG ATCC cells transfected with target specific siRNA probe

    Predicted band size: 26 kDa



    Loading control: anti- GAPDH

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)

    Immunohistochemical analysis of human skeletal muscle tissue labelling MIC19 with ab224565 at 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)

    Immunohistochemical analysis of human liver tissue labelling MIC19 with ab224565 at 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)

    Paraffin-embedded human stomach lower tissue stained for MIC19 using ab224565 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-MIC19 antibody (ab224565)
    Western blot - Anti-MIC19 antibody (ab224565)
    All lanes : Anti-MIC19 antibody (ab224565) at 1/100 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
    Lane 2 : NBT-II cell lysate

    Developed using the ECL technique.

    Predicted band size: 26 kDa

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab224565? Please let us know so that we can cite the reference in this datasheet.

    ab224565 has not yet been referenced specifically in any publications.

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