Recombinant
RabMAb

Recombinant Anti-MICA + MICB antibody [EPR22071-91] (ab222098)

Overview

  • Product name

    Anti-MICA + MICB antibody [EPR22071-91]
  • Description

    Rabbit monoclonal [EPR22071-91] to MICA + MICB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human MICB aa 1-300. The exact sequence is proprietary.
    Database link: Q29980

  • Positive control

    • WB: His-tagged MICA recombinant protein (aa24-307), His-tagged MICB (aa24-297; K562, HeLa, U266B1 and HUVEC whole cell lysates. IP: K562 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22071-91
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab222098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 43 kDa.
IP 1/30.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    Images

    • MICA + MICB were immunoprecipitated from 0.35 mg of K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab222098 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222098 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

      Lane 1: K562 whole cell lysate 10 μg (Input).
      Lane 2: ab222098 IP in K562 whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222098 in K562 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 10 seconds.

    • All lanes : Anti-MICA + MICB antibody [EPR22071-91] (ab222098) at 1/1000 dilution

      Lane 1 : U266B1 (human multiple myeloma b lymphocyte) whole cell lysate
      Lane 2 : HUVEC (human umbilical vein endothelial cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 43 kDa
      Observed band size: 50-60 kDa why is the actual band size different from the predicted?


      Exposure time: 103 seconds


      Blocking and dilution buffer: 5% NFDM/TBST .

      Bands between 50-60kDa represent the glycosylated form.
      The molecular weight observed is consistent with what has been described in the literature (PMID: 10359807; 19147769; 21631944).

    • All lanes : Anti-MICA + MICB antibody [EPR22071-91] (ab222098) at 1/1000 dilution

      Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
      Lane 2 : K562 whole cell lysate treated with PNGase F
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 4 : HeLa whole cell lysate treated with PNGase F

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 43 kDa


      Exposure time: 136 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

      Bands between 50-60kDa represent the glycosylated form. After PNGase F treatment, the de-glycosylated form appears around 40kDa.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 10359807; 19147769; 21631944, 19568411).

    • All lanes : Anti-MICA + MICB antibody [EPR22071-91] (ab222098) at 1/1000 dilution

      Lane 1 : His-tagged MICA recombinant protein was expressed in E. coli (aa24-307)
      Lane 2 : His-tagged MICB recombinant protein was expressed in HEK-293 (aa24-297

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 43 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

      The higher mass of MICB than that of MICA was due to glycosylation in the mammalian expression system.

    References

    ab222098 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab222098.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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